Detailed information of TA system TA163798

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-TIGR02293
• Location: 261492..262393
• Replicon: plasmid pRHBSTW-00310_2
• Accession: NZ_CP056623
• Organism: Citrobacter freundii strain RHBSTW-00310

Toxin (Protein)

• Gene name: -
• Uniprot ID: S1EZ83
• Locus tag: HV151_RS25715
• Protein ID: WP_008786589.1
• Coordinates: 261492..261950 (-)
• Length: 153 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: HV151_RS25720
• Protein ID: WP_008786588.1
• Coordinates: 261947..262393 (-)
• Length: 149 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV151_RS25670	257218..257460	+	243	WP_007372291.1	hypothetical protein	-
HV151_RS25675	257471..257869	+	399	WP_007372292.1	hypothetical protein	-
HV151_RS25680	257953..258279	+	327	WP_007372293.1	hypothetical protein	-
HV151_RS25685	258642..258980	-	339	WP_007372294.1	hypothetical protein	-
HV151_RS25690	259218..259661	+	444	WP_007372295.1	hypothetical protein	-
HV151_RS25695	259780..260121	+	342	WP_007372296.1	hypothetical protein	-
HV151_RS25700	260280..260834	+	555	WP_007372297.1	hypothetical protein	-
HV151_RS25705	260884..261150	+	267	WP_007372298.1	hypothetical protein	-
HV151_RS25710	261218..261448	-	231	WP_007372299.1	hypothetical protein	-
HV151_RS25715	261492..261950	-	459	WP_008786589.1	RES family NAD+ phosphorylase	Toxin
HV151_RS25720	261947..262393	-	447	WP_008786588.1	DUF2384 domain-containing protein	Antitoxin
HV151_RS25725	262542..262859	+	318	WP_016241555.1	hypothetical protein	-
HV151_RS25730	262901..263479	+	579	Protein_295	hypothetical protein	-
HV151_RS25735	263537..263824	-	288	WP_000323025.1	type II toxin-antitoxin system mRNA interferase RelE	-
HV151_RS25740	263824..264063	-	240	WP_004196370.1	type II toxin-antitoxin system antitoxin RelB	-
HV151_RS25745	264314..264679	+	366	WP_009651956.1	hypothetical protein	-
HV151_RS25750	264723..265460	+	738	WP_008460272.1	HupE/UreJ family protein	-
HV151_RS25755	265474..266163	+	690	WP_004196322.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	formA	-	1..312907	312907

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (3-148)

Antitoxin

• Antitoxin Xre/MbcA/ParS-like, toxin-binding domain (95-145)

Sequences

Toxin

Protein

• Length: 153 a.a.
• Molecular weight: 17005.43 Da
• Isoelectric Point: 4.5730

>T163798 WP_008786589.1 NZ_CP056623:c261950-261492 [Citrobacter freundii]
VILYRLTKTRYLMTAWSGLGAKETGGRWNSVGTAMVYLSETASLTMLETLVHIHAPQLLDDFTLLSLDVPDDQIQTFDMS
RLPDNWASEDAPAELALYGDNWAESGSSIALRIPSALSPVEFNYLLNPAHPELFDLMATVKKIPFRFDSRLK

Nucleotide

• Length: 459 bp

>T163798 NZ_CP072763:1239615-1239989 [Mycobacterium tuberculosis]
GTGATCCTGGTCGACACTTCGGTATGGATTGAGCACCTGCGCGCCGCCGACGCGCGACTCGTCGAGCTGCTGGGCGATGA
CGAGGCCGGTTGCCATCCGCTCGTCATCGAGGAGCTGGCGCTTGGCTCGATCAAGCAGCGAGACGTTGTTCTCGATCTGT
TGGCCAACCTCTACCAGTTTCCGGTGGTGACCCACGACGAAGTGTTGCGGCTTGTCGGTCGGCGGCGGTTGTGGGGTCGG
GGACTCGGTGCCGTCGATGCCAACCTTCTTGGTTCGGTGGCTCTGGTTGGCGGCGCGCGACTATGGACGCGGGACAAGCG
GTTGAAGGCGGCGTGCGCGGAAAGCGGTGTTGCGCTGGCTGAGGAAGTGTCCTGA

Antitoxin

Protein

• Length: 149 a.a.
• Molecular weight: 16689.02 Da
• Isoelectric Point: 6.9812

>AT163798 WP_008786588.1 NZ_CP056623:c262393-261947 [Citrobacter freundii]
MTMKVFSPDVTKTNQHCLWQVAGLDNDGIALMDKINHGLDGTVAKHISEWANITPSELRKMSGIPNTTFNRSIKDRFTAD
QSERLVRIIRVIERAVELFEGDKEAAHKWLNEANRGLSWKSPAELVSSETGALEVMRLITRIEHGVYS

Nucleotide

• Length: 447 bp

>AT163798 NZ_CP072763:1239421-1239618 [Mycobacterium tuberculosis]
ATGAGGACGACGGTGACCGTTGACGACGCCTTGTTAGCCAAAGCGGCCGAATTGACTGGGGTGAAAGAGAAGTCGACGCT
CCTGCGCGAGGGGTTGCAGACACTGGTCCGGGTGGAGAGCGCCCGGCGGTTGGCGGCTCTCGGCGGCACCGACCCGCAAG
CTACCGCGGCGCCGAGACGCCGGACGTCGCCCCGGTGA

Structures

Toxin

• AlphaFold DB: S1EZ83