Detailed information of TA system TA163783

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 1147108..1147787
• Replicon: chromosome
• Accession: NZ_CP056622
• Organism: Citrobacter freundii strain RHBSTW-00310

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A7D6YND6
• Locus tag: HV151_RS05475
• Protein ID: WP_032642920.1
• Coordinates: 1147108..1147404 (-)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A9K1QZR3
• Locus tag: HV151_RS05480
• Protein ID: WP_032642918.1
• Coordinates: 1147464..1147787 (-)
• Length: 108 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV151_RS05450	1142215..1144119	+	1905	WP_048232635.1	5-dehydro-2-deoxygluconokinase	-
HV151_RS05455	1144134..1145024	+	891	WP_048232633.1	myo-inosose-2 dehydratase	-
HV151_RS05460	1145034..1145852	+	819	WP_048232631.1	5-deoxy-glucuronate isomerase	-
HV151_RS05465	1146067..1146432	-	366	Protein_1067	autotransporter outer membrane beta-barrel domain-containing protein	-
HV151_RS05470	1146440..1146601	-	162	Protein_1068	integrase arm-type DNA-binding domain-containing protein	-
HV151_RS05475	1147108..1147404	-	297	WP_032642920.1	TA system toxin CbtA family protein	Toxin
HV151_RS05480	1147464..1147787	-	324	WP_032642918.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
HV151_RS05485	1147809..1148030	-	222	WP_032642916.1	DUF987 domain-containing protein	-
HV151_RS05490	1148039..1148509	-	471	WP_032642914.1	DNA repair protein RadC	-
HV151_RS05495	1148542..1149363	-	822	WP_032642912.1	DUF945 domain-containing protein	-
HV151_RS05500	1149584..1150024	-	441	WP_135695985.1	NirD/YgiW/YdeI family stress tolerance protein	-
HV151_RS05505	1150185..1151327	-	1143	WP_029602872.1	nickel/cobalt efflux protein RcnA	-
HV151_RS05510	1151340..1151609	-	270	WP_009653703.1	metal-sensing transcriptional repressor	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	galE / acrB	1050007..1181871	131864

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (4-97)

Antitoxin

• Antitoxin CbeA (4-102)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11228.87 Da
• Isoelectric Point: 6.0673

>T163783 WP_032642920.1 NZ_CP056622:c1147404-1147108 [Citrobacter freundii]
VPVSSRLSPVMVWQQLLTYLLEHHYGLTLNDTPFHDDTAIEEHIDAGITLADAVNFLVEKYELARIDRKGFTWREQPPFI
TATDILRAKKNIGLLPRS

Nucleotide

• Length: 297 bp

>T163783 NZ_CP072763:677925-678332 [Mycobacterium tuberculosis]
GTGATCATCGACACGAGTGCGCTGCTTGCCTATTTCGACGCCGCCGAGCCAGACCACGCCGCAGTGTCTGAGTGCATCGA
TAGCTCCGCAGACGCGCTCGTCGTATCCCCTTATGTGGTAGCGGAACTCGACTATCTCGTCGCCACCCGGGTAGGTGTCG
ATGCCGAGCTCGCCGTCCTGCGTGAACTCGCCGGCGGGGCCTGGGAGCTCGCCAACTGCGGTGCCGCCGAAATCGAGCAG
GCCGCCCGCATCGTCACGAAATACCAGGATCAGCGGATCGGGATCGCGGATGCGGCCAACGTCGTGCTGGCCGACCGATA
CCGCACGCGCACGATCCTCACCCTGGACCGTCGGCACTTCTCGGCGCTGCGGCCGATCGGCGGTGGGCGCTTCACCGTCA
TTCCGTAA

Antitoxin

Protein

• Length: 108 a.a.
• Molecular weight: 12082.82 Da
• Isoelectric Point: 6.6250

>AT163783 WP_032642918.1 NZ_CP056622:c1147787-1147464 [Citrobacter freundii]
MQNSWGLKRDISPCFGARLVQEGCRLHFLADRAGISGTFNEEEALRLDQAFPLVMKQLELMLTTGELNPRHQHCVTLYHN
GLTCEADTLGSHGYVYIVIYPQAFATR

Nucleotide

• Length: 324 bp

>AT163783 NZ_CP072763:677713-677928 [Mycobacterium tuberculosis]
GTGGACAAGACGACGGTCTACCTGCCGGATGAACTCAAGGCGGCCGTGAAGCGCGCCGCTCGGCAGCGCGGAGTCTCCGA
AGCGCAGGTAATCCGGGAGTCCATCCGGGCGGCGGTCGGCGGCGCCAAGCCGCCGCCGCGCGGGGGTCTATATGCGGGTT
CGGAGCCCATCGCGCGGCGAGTCGACGAGCTGCTGGCTGGCTTCGGTGAGCGGTGA

Structures

Toxin

• AlphaFold DB: A0A7D6YND6