Detailed information of TA system TA163778

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 4198539..4199055
• Replicon: chromosome
• Accession: NZ_CP056597
• Organism: Citrobacter freundii strain RHBSTW-00334

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A5I6CHB9
• Locus tag: HV164_RS19895
• Protein ID: WP_000220581.1
• Coordinates: 4198539..4198823 (-)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: HV164_RS19900
• Protein ID: WP_115259576.1
• Coordinates: 4198813..4199055 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV164_RS19880	4193740..4195392	+	1653	WP_181625620.1	alpha,alpha-phosphotrehalase	-
HV164_RS19885	4195811..4197949	+	2139	WP_115259574.1	anaerobic ribonucleoside-triphosphate reductase	-
HV164_RS19890	4198071..4198535	+	465	WP_096759476.1	anaerobic ribonucleoside-triphosphate reductase-activating protein	-
HV164_RS19895	4198539..4198823	-	285	WP_000220581.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HV164_RS19900	4198813..4199055	-	243	WP_115259576.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
HV164_RS19905	4199133..4201046	-	1914	WP_117342405.1	BglG family transcription antiterminator	-
HV164_RS19910	4201068..4201808	-	741	WP_181625621.1	KDGP aldolase family protein	-
HV164_RS19915	4201805..4202923	-	1119	WP_096758517.1	DgaE family pyridoxal phosphate-dependent ammonia lyase	-
HV164_RS19920	4202907..4204040	-	1134	WP_181625622.1	amidohydrolase/deacetylase family metallohydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-83)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-80)

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 10868.70 Da
• Isoelectric Point: 10.0482

>T163778 WP_000220581.1 NZ_CP056597:c4198823-4198539 [Citrobacter freundii]
MTYELEFDPRALKEWHKLGDTVKAQLKKKLADVLLNPRIDSARLNGLPDCYKIKLKSSGYRLVYQVRDEVVIVFVVAVGK
REHSAVYHDANKRL

Nucleotide

• Length: 285 bp

>T163778 NZ_CP072763:363476-363778 [Mycobacterium tuberculosis]
TTGATCGCTCCCGGCGACATCGCGCCGCGCCGCGACAGTGAACACGAGCTCTACGTCGCCGTCTTGTCCAACGCGCTCCA
TCGGGCCGCGGACACCGGACGGGTGATCACCTGCCCATTCATTCCGGGCCGGGTCCCCGAGGATCTCTTGGCGATGGTGG
TGGCGGTCGAGCAACCCAACGGCACGCTGCTGCCGGAACTCGTGCAGTGGCTTCATGTTGCCGCGCTCGGTGCGCCACTC
GGCAACGCGGGCGTGGCCGCCCTACGCGAGGCTGCCTCGGTCGTGACAGCTCTGCTCTGTTAG

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8947.32 Da
• Isoelectric Point: 5.0490

>AT163778 WP_115259576.1 NZ_CP056597:c4199055-4198813 [Citrobacter freundii]
MATLNVRLDDKLKNEAYAVLDKLNITPTEAVRLLFQYVAETGRMPVKTVTLSDSEDALIQTVRERLSSPQKGIKVSLDDL

Nucleotide

• Length: 243 bp

>AT163778 NZ_CP072763:363252-363479 [Mycobacterium tuberculosis]
ATGACGAAAGAGAAGATCTCCGTGACGGTGGACGCGGCCGTCCTCGCGGCGATCGACGCGGACGCCAGGGCGGCGGGTTT
GAATCGGTCGGAAATGATTGAGCAGGCACTGCGCAACGAGCACCTGCGTGTCGCTCTGCGCGATTACACGGCTAAAACCG
TACCGGCGTTGGACATCGATGCCTACGCACAGCGGGTGTACCAGGCGAACCGGGCGGCCGGAAGTTGA

Structures

Toxin

• AlphaFold DB: A0A5I6CHB9