Detailed information of TA system TA163775

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 3590611..3591231
• Replicon: chromosome
• Accession: NZ_CP056597
• Organism: Citrobacter freundii strain RHBSTW-00334

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: R8WYV2
• Locus tag: HV164_RS17125
• Protein ID: WP_002892050.1
• Coordinates: 3591013..3591231 (+)
• Length: 73 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: -
• Locus tag: HV164_RS17120
• Protein ID: WP_115259201.1
• Coordinates: 3590611..3590985 (+)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV164_RS17110	3585757..3586950	+	1194	WP_096758038.1	multidrug efflux RND transporter periplasmic adaptor subunit AcrA	-
HV164_RS17115	3586973..3590122	+	3150	WP_096758039.1	efflux RND transporter permease AcrB	-
HV164_RS17120	3590611..3590985	+	375	WP_115259201.1	Hha toxicity modulator TomB	Antitoxin
HV164_RS17125	3591013..3591231	+	219	WP_002892050.1	hemolysin expression modulator Hha	Toxin
HV164_RS17130	3591613..3592086	+	474	WP_181539966.1	YlaC family protein	-
HV164_RS17135	3592160..3592300	-	141	WP_005125190.1	type B 50S ribosomal protein L36	-
HV164_RS17140	3592302..3592562	-	261	WP_115259215.1	type B 50S ribosomal protein L31	-
HV164_RS17145	3592747..3594300	+	1554	WP_181625553.1	EAL domain-containing protein	-
HV164_RS17150	3594354..3594707	-	354	WP_115259217.1	DUF1428 family protein	-
HV164_RS17155	3594790..3595401	-	612	WP_174360590.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (13-69)

Antitoxin

• Biofilm formation regulator YbaJ (1-116)

Sequences

Toxin

Protein

• Length: 73 a.a.
• Molecular weight: 8628.00 Da
• Isoelectric Point: 8.9008

>T163775 WP_002892050.1 NZ_CP056597:3591013-3591231 [Citrobacter freundii]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Nucleotide

• Length: 219 bp

>T163775 NZ_CP072762:c3821201-3820809 [Mycobacterium tuberculosis]
ATGGCGGCCATCTATCTCGACTCGTCCGCGATCGTCAAGCTCGCGGTTCGCGAGCCAGAGTCGGACGCGCTGCGCCGCTA
CCTTCGCACCCGCCACCCGCGAGTGTCGAGTGCCCTTGCGCGCGCGGAGGTCATGCGGGCCCTGCTTGACAAGGGTGAAT
CAGCGCGGAAAGCCGGCCGCCGGGCGCTGGCTCATCTCGATCTGCTGCGCGTCGACAAGCGCGTCCTCGATCTCGCTGGT
GGGCTGCTGCCATTCGAGCTGCGCACGCTCGATGCCATCCACTTGGCGACGGCGCAGCGGCTCGGCGTCGATCTGGGCCG
GTTGTGCACCTACGACGACCGGATGCGCGACGCCGCCAAGACGCTCGGCATGGCCGTGATCGCCCCGTCGTGA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 14454.26 Da
• Isoelectric Point: 5.5653

>AT163775 WP_115259201.1 NZ_CP056597:3590611-3590985 [Citrobacter freundii]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEQSNHGWVNDPTSATSLQLNELIEHIATFALNYKIKYNEDNKLIAQIDEYL
DDTFMLFSNYGINTQDLQKWRKSGNRLFRCFVNATRANPVSLSC

Nucleotide

• Length: 375 bp

>AT163775 NZ_CP072762:c3821476-3821201 [Mycobacterium tuberculosis]
ATGACCTCGGTGGGGGTGCGTGCGCTGCGGCAGCGGGCCAGCGAGCTGCTGCGCCGAGTCGAGGCCGGCGAGACGATCGA
GATCACCGACCGCGGCCGGCCCGTCGCCCTGCTCTCGCCGCTGCCGCAGGGCGGTCCATACGAGCAGCTGCTGGCAAGCG
GCGAGATCGAGCGTGCCACGCTCGACGTCGTTGATCTGCCCGAACCGCTCGACCTCGACGCGGGCGTCGAGCTGCCGTCG
GTGACGCTTGCGCGCCTGCGTGAGCACGAGCGTTGA

Structures

Toxin

• AlphaFold DB: A0A2P8K6F2