Detailed information of TA system TA163774

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 2937681..2938319
• Replicon: chromosome
• Accession: NZ_CP056597
• Organism: Citrobacter freundii strain RHBSTW-00334

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A8H9UJ60
• Locus tag: HV164_RS14045
• Protein ID: WP_019077450.1
• Coordinates: 2937681..2937863 (+)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: HV164_RS14050
• Protein ID: WP_181625487.1
• Coordinates: 2937888..2938319 (+)
• Length: 144 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV164_RS14000	2934017..2934499	-	483	WP_181625479.1	phage terminase small subunit P27 family	-
HV164_RS14005	2934656..2935006	-	351	WP_181625480.1	HNH endonuclease	-
HV164_RS14010	2934994..2935209	-	216	WP_181625481.1	hypothetical protein	-
HV164_RS14015	2935447..2935932	+	486	WP_181625482.1	GNAT family N-acetyltransferase	-
HV164_RS14020	2935936..2936139	-	204	WP_181625891.1	hypothetical protein	-
HV164_RS14025	2936153..2936299	+	147	WP_181625483.1	hypothetical protein	-
HV164_RS14030	2936361..2936906	-	546	WP_181625484.1	glycoside hydrolase family 108 protein	-
HV164_RS14035	2936881..2937186	-	306	WP_181625485.1	phage holin family protein	-
HV164_RS14040	2937176..2937562	-	387	WP_181625486.1	holin	-
HV164_RS14045	2937681..2937863	+	183	WP_019077450.1	type II toxin-antitoxin system HicA family toxin	Toxin
HV164_RS14050	2937888..2938319	+	432	WP_181625487.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
HV164_RS14055	2938569..2939453	+	885	WP_181625488.1	hypothetical protein	-
HV164_RS14060	2939488..2939832	-	345	WP_048235572.1	antitermination protein	-
HV164_RS14065	2939850..2940839	-	990	WP_181625489.1	DUF968 domain-containing protein	-
HV164_RS14070	2940847..2941656	-	810	WP_181625490.1	KilA-N domain-containing protein	-
HV164_RS14075	2941676..2942065	-	390	WP_181625491.1	RusA family crossover junction endodeoxyribonuclease	-
HV164_RS14080	2942062..2942388	-	327	WP_181625492.1	LexA family transcriptional regulator	-
HV164_RS14085	2942385..2942864	-	480	WP_181625493.1	PerC family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2886008..2970663	84655

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-60)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (7-137)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6561.76 Da
• Isoelectric Point: 10.7014

>T163774 WP_019077450.1 NZ_CP056597:2937681-2937863 [Citrobacter freundii]
MKSTDLIKELTAAGCELKRHNGGSHQIWWSPITGKTFPVPHPKKDLPLGTVKSIKKLAGI

Nucleotide

• Length: 183 bp

>T163774 NZ_CP072762:3792602-3792859 [Mycobacterium tuberculosis]
GTGAGAAGCGTCAACTTCGATCCCGATGCCTGGGAGGACTTCTTGTTCTGGCTGGCCGCTGATCGCAAAACGGCCCGTCG
GATCACCCGGTTGATCGGAGAAATTCAGCGTGATCCGTTCAGCGGGATCGGCAAACCCGAGCCGCTCCAAGGTGAGTTGT
CGGGATACTGGTCGCGCCGGATCGACGACGAACACCGGCTGGTGTATCGAGCGGGCGACGACGAAGTCACGATGCTGAAG
GCCCGATACCACTACTGA

Antitoxin

Protein

• Length: 144 a.a.
• Molecular weight: 16192.30 Da
• Isoelectric Point: 4.3006

>AT163774 WP_181625487.1 NZ_CP056597:2937888-2938319 [Citrobacter freundii]
MFFSVGVETPKDENTAYGMIVPAFSAYDYGCFSAADTQDQIAPMVKEAILMVVEDLIQNKNISVEDIKDAGYLVYSLNPE
YKDFDSWVMVDVDLSEFEGKPQRINISLPDTLIKRIDNAVKASNVYRDRSHFIAEAARHELKQ

Nucleotide

• Length: 432 bp

>AT163774 NZ_CP072762:3792330-3792605 [Mycobacterium tuberculosis]
ATGAGCATCAGTGCGAGCGAGGCGAGGCAGCGCCTGTTTCCACTCATCGAACAGGTCAATACCGATCACCAGCCGGTGCG
GATCACCTCCCGGGCCGGCGATGCGGTGCTGATGTCCGCCGACGACTACGACGCGTGGCAGGAAACGGTCTATCTGCTGC
GCTCACCGGAGAACGCCAGGCGGTTGATGGAAGCGGTTGCCCGGGATAAGGCTGGGCACTCGGCTTTCACCAAGTCTGTA
GATGAGCTGCGGGAGATGGCCGGCGGCGAGGAGTGA