Detailed information of TA system TA163761

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-RelB
• Location: 249023..249609
• Replicon: chromosome
• Accession: NZ_CP056597
• Organism: Citrobacter freundii strain RHBSTW-00334

Toxin (Protein)

• Gene name: doc
• Uniprot ID: -
• Locus tag: HV164_RS01200
• Protein ID: WP_181625665.1
• Coordinates: 249241..249609 (+)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: -
• Locus tag: HV164_RS01195
• Protein ID: WP_096759232.1
• Coordinates: 249023..249244 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV164_RS01170	244080..245189	+	1110	WP_115257271.1	high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK	-
HV164_RS01175	245237..246163	+	927	WP_096759229.1	high-affinity branched-chain amino acid ABC transporter permease LivH	-
HV164_RS01180	246160..247440	+	1281	WP_096759230.1	branched chain amino acid ABC transporter permease LivM	-
HV164_RS01185	247437..248204	+	768	WP_181540283.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG	-
HV164_RS01190	248222..248935	+	714	WP_096759502.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF	-
HV164_RS01195	249023..249244	+	222	WP_096759232.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
HV164_RS01200	249241..249609	+	369	WP_181625665.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
HV164_RS01205	249864..251180	+	1317	WP_096759233.1	sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB	-
HV164_RS01210	251285..252172	+	888	WP_096759234.1	sn-glycerol-3-phosphate ABC transporter permease UgpA	-
HV164_RS01215	252169..253014	+	846	WP_174361255.1	sn-glycerol-3-phosphate ABC transporter permease UgpE	-
HV164_RS01220	253016..254086	+	1071	WP_117342478.1	sn-glycerol-3-phosphate import ATP-binding protein UgpC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	247437..254826	7389

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Fido domain (24-86)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-50)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13741.04 Da
• Isoelectric Point: 6.7255

>T163761 WP_181625665.1 NZ_CP056597:249241-249609 [Citrobacter freundii]
MTLQIISAEEIIQFHDRLLRMTPGVTGMPDPGRAEALMYRVLNQIEYEDVTDVWLLAAMHLLAISRGYIFNDGNKRTALF
ITLLFLKRNGIALSANPDFVAMTVEAAAGRLSLEQIAHRLRQ

Nucleotide

• Length: 369 bp

>T163761 NZ_CP072762:2890371-2890748 [Mycobacterium tuberculosis]
GTGAAGCTGATCGACACCACCATCGCGGTCGACCACCTTCGCGGCGAACCCGCGGCAGCCGTGCTGCTCGCCGAACTGAT
AAACAACGGTGAGGAGATCGCGGCCAGCGAGCTGGTCCGATTCGAACTCCTCGCCGGTGTGCGGGAAAGCGAACTCGCGG
CGCTCGAGGCCTTCTTCTCGGCAGTGGTGTGGACCCTGGTGACCGAGGACATTGCCCGGATCGGCGGACGACTCGCCCGT
CGATACCGGTCCAGCCACCGCGGTATCGACGACGTGGACTACCTGATCGCTGCGACCGCCATTGTGGTCGACGCCGACCT
GCTCACCACCAATGTGCGCCACTTCCCGATGTTCCCGGATCTGCAGCCGCCGTACTGA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8260.32 Da
• Isoelectric Point: 4.7769

>AT163761 WP_096759232.1 NZ_CP056597:249023-249244 [Citrobacter freundii]
MRTVNYSEARQNLAEVLDSAVTGVPVTITRRGHKPAVIISAEEFERYQAARLDDEFADIMMIHGNEIRELADK

Nucleotide

• Length: 222 bp

>AT163761 NZ_CP072762:2890117-2890374 [Mycobacterium tuberculosis]
ATGCGGACTCAGGTGACCCTGGGCAAAGAGGAGCTTGAGCTGCTCGATCGTGCCGCCAAGGCGAGTGGCGCATCGCGGTC
CGAACTCATCCGACGCGCAATTCACCGTGCCTACGGGACTGGATCCAAGCAGGAACGGCTCGCCGCGCTCGACCACAGCC
GTGGCTCGTGGCGAGGACGGGACTTCACCGGCACCGAGTATGTCGACGCCATTCGGGGCGACCTCAACGAACGACTTGCT
CGGCTCGGTCTGGCGTGA