163760

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 4860663..4861279 Replicon chromosome
Accession NZ_CP056595
Organism Citrobacter freundii strain RHBSTW-00342

Toxin (Protein)


Gene name vapC Uniprot ID A0A0J1MQ96
Locus tag HV167_RS23350 Protein ID WP_003028682.1
Coordinates 4860663..4861037 (-) Length 125 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A6B5NTK4
Locus tag HV167_RS23355 Protein ID WP_043018956.1
Coordinates 4861037..4861279 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HV167_RS23335 4858166..4859068 + 903 WP_003028691.1 formate dehydrogenase O subunit beta -
HV167_RS23340 4859065..4859700 + 636 WP_003028686.1 formate dehydrogenase cytochrome b556 subunit -
HV167_RS23345 4859697..4860626 + 930 WP_071696217.1 formate dehydrogenase accessory protein FdhE -
HV167_RS23350 4860663..4861037 - 375 WP_003028682.1 type II toxin-antitoxin system VapC family toxin Toxin
HV167_RS23355 4861037..4861279 - 243 WP_043018956.1 ribbon-helix-helix domain-containing protein Antitoxin
HV167_RS23360 4861485..4862393 + 909 WP_071696216.1 alpha/beta hydrolase -
HV167_RS23365 4862544..4863485 - 942 WP_003825286.1 fatty acid biosynthesis protein FabY -
HV167_RS23370 4863530..4863967 - 438 WP_003028671.1 D-tyrosyl-tRNA(Tyr) deacylase -
HV167_RS23375 4863964..4864836 - 873 WP_003028669.1 virulence factor BrkB family protein -
HV167_RS23380 4864830..4865429 - 600 WP_003028663.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Ribbon-helix-helix protein, CopG

(13-47)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13674.89 Da        Isoelectric Point: 9.5622

>T163760 WP_003028682.1 NZ_CP056595:c4861037-4860663 [Citrobacter freundii]
MVKGSALFDTNILIDLFSGRNEAKLAIETWPPQNAISLITWMEVMVGAKKYHQEARTRVALGAFNVIGVSQDIAERSVSI
RQEYGMKLPDAIILATAQIHRLTLVTRNTKDFAGLSGVVTPYTL

Download         Length: 375 bp

>T163760 NZ_CP072762:2889665-2890078 [Mycobacterium tuberculosis]
ATGGTGTTCTGCGTCGACACCAGCGCGTGGCATCACGCGGCGCGGCCGGAAGTTGCGCGCCGATGGTTGGCGGCCTTGTC
CGCGGACCAGATCGGCATCTGCGACCACGTGCGGTTGGAGATCCTGTACTCGGCGAACTCCGCTACCGACTACGACGCGC
TCGCCGACGAACTCGACGGCTTGGCCCGTATACCAGTCGGTGCCGAAACCTTTACGCGCGCATGCCAAGTCCAGCGTGAG
CTTGCCCACGTCGCCGGTCTGCATCACCGCAGCGTGAAGATCGCCGATCTTGTCATCGCCGCGGCGGCCGAACTTTCAGG
CACCATCGTGTGGCATTACGACGAGAACTATGACCGGGTCGCCGCCATCACCGGCCAACCTACGGAGTGGATCGTGCCGC
GCGGGACCCTTTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9451.75 Da        Isoelectric Point: 4.3437

>AT163760 WP_043018956.1 NZ_CP056595:c4861279-4861037 [Citrobacter freundii]
MMAGMDMGRILLDLSDEVIKRLDDLKVQRNLPRAELLREAVEQYLERQDQAETTISRALGLWQGCEEDGVEYQRKLREEW

Download         Length: 243 bp

>AT163760 NZ_CP072762:2890117-2890374 [Mycobacterium tuberculosis]
ATGCGGACTCAGGTGACCCTGGGCAAAGAGGAGCTTGAGCTGCTCGATCGTGCCGCCAAGGCGAGTGGCGCATCGCGGTC
CGAACTCATCCGACGCGCAATTCACCGTGCCTACGGGACTGGATCCAAGCAGGAACGGCTCGCCGCGCTCGACCACAGCC
GTGGCTCGTGGCGAGGACGGGACTTCACCGGCACCGAGTATGTCGACGCCATTCGGGGCGACCTCAACGAACGACTTGCT
CGGCTCGGTCTGGCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J1MQ96

Antitoxin

Source ID Structure
AlphaFold DB A0A6B5NTK4

References