Detailed information of TA system TA163757

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4541192..4541945
• Replicon: chromosome
• Accession: NZ_CP056595
• Organism: Citrobacter freundii strain RHBSTW-00342

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: R8WN26
• Locus tag: HV167_RS21845
• Protein ID: WP_016154457.1
• Coordinates: 4541568..4541945 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: HV167_RS21840
• Protein ID: WP_162838704.1
• Coordinates: 4541192..4541518 (+)
• Length: 109 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV167_RS21780	4536251..4536517	+	267	WP_032950091.1	hypothetical protein	-
HV167_RS21785	4536530..4536841	+	312	WP_009652410.1	hypothetical protein	-
HV167_RS21790	4536888..4537358	+	471	WP_009652431.1	hypothetical protein	-
HV167_RS21795	4537407..4537919	+	513	WP_032950094.1	DUF4234 domain-containing protein	-
HV167_RS21800	4538001..4538222	+	222	WP_009652443.1	hypothetical protein	-
HV167_RS21805	4538278..4538511	+	234	WP_009652474.1	DUF905 domain-containing protein	-
HV167_RS21810	4538615..4539433	+	819	WP_009652424.1	DUF945 domain-containing protein	-
HV167_RS21815	4539433..4539687	+	255	WP_009652519.1	hypothetical protein	-
HV167_RS21820	4539716..4540177	+	462	WP_045890390.1	antirestriction protein	-
HV167_RS21825	4540188..4540406	+	219	WP_009652503.1	hypothetical protein	-
HV167_RS21830	4540418..4540900	+	483	WP_016154455.1	DNA repair protein RadC	-
HV167_RS21835	4540914..4541135	+	222	WP_009652392.1	DUF987 domain-containing protein	-
HV167_RS21840	4541192..4541518	+	327	WP_162838704.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
HV167_RS21845	4541568..4541945	+	378	WP_016154457.1	TA system toxin CbtA family protein	Toxin
HV167_RS21850	4541942..4542433	+	492	WP_009652438.1	hypothetical protein	-
HV167_RS21855	4542463..4542666	+	204	WP_009652505.1	DUF957 domain-containing protein	-
HV167_RS21860	4542747..4543592	+	846	Protein_4287	DUF4942 domain-containing protein	-
HV167_RS21865	4543604..4543912	+	309	WP_181558310.1	hypothetical protein	-
HV167_RS21870	4544028..4545281	+	1254	WP_001218761.1	integrase arm-type DNA-binding domain-containing protein	-
HV167_RS21875	4545432..4546099	-	668	Protein_4290	IS1 family transposase	-
HV167_RS21880	4546112..4546402	+	291	Protein_4291	IS6-like element IS26 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (4-103)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14168.19 Da
• Isoelectric Point: 9.5224

>T163757 WP_016154457.1 NZ_CP056595:4541568-4541945 [Citrobacter freundii]
MQTQPVPPKREVSPRPSPVAIWQRLLSHLLDRHYGLTLNDTPFGKDGVIQEHIDAGISLCDAVNFIVEKYDLVRTDRRGF
NAETQSPLLTSIDILRARKATGLMTRHSYRAVTDITTGKYREVQP

Nucleotide

• Length: 378 bp

>T163757 NZ_CP072762:c2405441-2405124 [Mycobacterium tuberculosis]
ATGACGCGCAGGCTGCGCGTCCATAACGGGGTTGAAGACGACCTATTCGAGGCGTTTTCCTACTACGCGGACGCGGCGCC
AGATCAGATCGATCGGCTTTACAACTTGTTTGTCGATGCCGTGACGAAGCGGATTCCGCAGGCTCCGAACGCGTTTGCGC
CGTTATTCAAGCACTATCGACACATCTACCTCCGGCCCTTCAGGTACTACGTTGCCTATCGGACGACCGACGAGGCTATC
GACATACTGGCTGTTCGGCACGGAATGGAGAACCCGAACGCGGTCGAGGCTGAGATCTCTGGCCGCACCTTCGAGTGA

Antitoxin

Protein

• Length: 109 a.a.
• Molecular weight: 12079.85 Da
• Isoelectric Point: 6.6283

>AT163757 WP_162838704.1 NZ_CP056595:4541192-4541518 [Citrobacter freundii]
IAAPWWGLKRDITPCFGARLVQEGNRLHYLNDRASITGTFSDADLRHLDQAFPLLLKQLELMLLSGELNPRHQHCVTLYA
KGLICEADSLGSHGYVYLAIYPTPATTE

Nucleotide

• Length: 327 bp

>AT163757 NZ_CP072762:c2405653-2405438 [Mycobacterium tuberculosis]
GTGGTGGTCAACCGGGCATTGCTGGCGAGCGTCGACGCACTGTCGCGTGATGAGCAGATTGAGCTCGTCGAGCACATCAA
CGGAAACCTAGCCGAGGGCATGCATATCAGCGAGGCCAACCAGGCGCTCATCGAAGCGCGGGCCAATGACACCGACGATG
CTCATTGGTCCACCATTGATGACTTCGACAAGCGGATCCGCGCCCGGCTCGGATGA

Structures

Toxin

• AlphaFold DB: R8WN26