Detailed information of TA system TA163756

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yfjZ-ypjF/CbtA-CbeA
• Location: 4261365..4262056
• Replicon: chromosome
• Accession: NZ_CP056595
• Organism: Citrobacter freundii strain RHBSTW-00342

Toxin (Protein)

• Gene name: ypjF
• Uniprot ID: -
• Locus tag: HV167_RS20485
• Protein ID: WP_071696537.1
• Coordinates: 4261365..4261694 (-)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: A0A2Z3WZC0
• Locus tag: HV167_RS20490
• Protein ID: WP_032410777.1
• Coordinates: 4261715..4262056 (-)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV167_RS20465	4257850..4258212	+	363	WP_071696540.1	endoribonuclease SymE	-
HV167_RS20470	4258264..4258899	-	636	WP_003837147.1	HNH endonuclease	-
HV167_RS20475	4259040..4259912	+	873	WP_081368679.1	HNH endonuclease	-
HV167_RS20480	4260093..4261100	-	1008	WP_071696538.1	restriction endonuclease	-
HV167_RS20485	4261365..4261694	-	330	WP_071696537.1	TA system toxin CbtA family protein	Toxin
HV167_RS20490	4261715..4262056	-	342	WP_032410777.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
HV167_RS20495	4262067..4262609	-	543	WP_071696536.1	DNA repair protein RadC	-
HV167_RS20500	4262622..4263065	-	444	WP_071696535.1	antirestriction protein	-
HV167_RS20505	4263096..4263917	-	822	WP_071696534.1	DUF945 domain-containing protein	-
HV167_RS20510	4264038..4264511	-	474	WP_071696533.1	hypothetical protein	-
HV167_RS20515	4264583..4265035	-	453	WP_081368678.1	hypothetical protein	-
HV167_RS20520	4265071..4265787	-	717	WP_024360475.1	WYL domain-containing protein	-
HV167_RS20525	4266031..4266906	-	876	WP_071696531.1	GTPase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-106)

Antitoxin

• Antitoxin CbeA (13-111)

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 12226.02 Da
• Isoelectric Point: 7.1639

>T163756 WP_071696537.1 NZ_CP056595:c4261694-4261365 [Citrobacter freundii]
MKTLPATNPQAANLCLSPVAVWQMLLARLLEQHYGLTLNDTTFSDETVIQKHIDAGISLADAVNFLVEKYGLVRIDRRGF
DWQEQSPYLRAVDILRARQTTGLLNSAAR

Nucleotide

• Length: 330 bp

>T163756 NZ_CP072762:c2367451-2367017 [Mycobacterium tuberculosis]
ATGAAGATCGTCGACGCGAACGTCTTGCTCTACGCCGTGAACACCACAAGTGAGCACCACAAGCCGTCGCTGCGCTGGCT
TGACGGTGCGCTGTCGGGCGCCGACCGCGTCGGGTTCGCCTGGGTGCCGTTGTTGGCGTTCGTGCGATTGGCGACCAAGG
TGGGGTTGTTCCCCCGTCCGCTTCCGCGGGAGGCGGCCATCACCCAGGTCGCGGATTGGCTAGCCGCACCCAGCGCCGTC
TTGGTGAATCCGACCGTCCGGCACGCCGATATCCTGGCGAGAATGCTGACGTACGTGGGAACCGGTGCCAACCTGGTCAA
CGACGCGCATCTGGCCGCGCTTGCCGTCGAGCATCGCGCCAGCATCGTGTCCTACGACAGTGACTTCGGCCGATTCGAAG
GGGTGCGCTGGGATCAGCCGCCCGCGCTGTTGTGA

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 12765.40 Da
• Isoelectric Point: 7.0269

>AT163756 WP_032410777.1 NZ_CP056595:c4262056-4261715 [Citrobacter freundii]
MKSTDSENDSFLKWGLNRNVTPCFGARLVQEGNRLHYLADRASITGKFSEAECLKLDVAFPHFISQMESMLTTGEMNPRH
AHRVTLYHNGFTCEADTLGSCGYVYIAVYSTHR

Nucleotide

• Length: 342 bp

>AT163756 NZ_CP072762:c2367712-2367458 [Mycobacterium tuberculosis]
ATGCGGACGACTGTCACATTGGATGACGACGTCGAGCAGTTGGTAAGGCGCCGGATGGCCGAGCGGCAAGTGTCCTTCAA
GAAGGCCCTCAACGACGCGATTCGCGACGGCGCCTCCGGGCGACCTGCGCCGAGTCACTTCAGCACCCGCACGGCAGACT
TGGGCGTCCCTGCGGTCAACCTCGACCGGGCCTTGCAGCTTGCGGCCGACCTCGAGGACGAAGAGCTTGTGCGCCGTCAA
CGCCGCGGGTCATAG

Structures

Antitoxin

• AlphaFold DB: A0A2Z3WZC0