Detailed information of TA system TA163755

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yafW-ykfI/CbtA-CbeA
• Location: 3836276..3836955
• Replicon: chromosome
• Accession: NZ_CP056595
• Organism: Citrobacter freundii strain RHBSTW-00342

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: A0A1B7JLK7
• Locus tag: HV167_RS18520
• Protein ID: WP_003031349.1
• Coordinates: 3836614..3836955 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yafW
• Uniprot ID: A0A1B7JLJ2
• Locus tag: HV167_RS18515
• Protein ID: WP_003031347.1
• Coordinates: 3836276..3836593 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV167_RS18475	3831294..3831734	+	441	WP_181558282.1	hypothetical protein	-
HV167_RS18480	3831758..3832227	+	470	Protein_3626	hypothetical protein	-
HV167_RS18485	3832833..3833183	-	351	WP_003036925.1	Morphinone reductase	-
HV167_RS18490	3833532..3834512	-	981	WP_032216024.1	IS5-like element ISKpn26 family transposase	-
HV167_RS18495	3834870..3835061	+	192	Protein_3629	DUF905 family protein	-
HV167_RS18500	3835075..3835536	+	462	WP_003031344.1	antirestriction protein	-
HV167_RS18505	3835552..3836028	+	477	WP_003031345.1	RadC family protein	-
HV167_RS18510	3836037..3836258	+	222	WP_003031346.1	DUF987 domain-containing protein	-
HV167_RS18515	3836276..3836593	+	318	WP_003031347.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
HV167_RS18520	3836614..3836955	+	342	WP_003031349.1	type IV toxin-antitoxin system toxin YkfI	Toxin
HV167_RS18530	3837535..3838788	-	1254	WP_071696411.1	glutamate-5-semialdehyde dehydrogenase	-
HV167_RS18535	3838800..3839903	-	1104	WP_003031373.1	glutamate 5-kinase	-
HV167_RS18540	3840194..3841249	+	1056	WP_003031375.1	phosphoporin PhoE	-
HV167_RS18545	3841305..3841706	-	402	WP_071696410.1	sigma factor-binding protein Crl	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	3833532..3834512	980

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (5-104)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12921.01 Da
• Isoelectric Point: 9.6543

>T163755 WP_003031349.1 NZ_CP056595:3836614-3836955 [Citrobacter freundii]
MKTLPATTQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAVDILRARQATGLLRQSRNNVVR

Nucleotide

• Length: 342 bp

>T163755 NZ_CP072762:2323988-2324398 [Mycobacterium tuberculosis]
TTGGCTGAGCCACGGCGAGGAGACCTTTGGCTGGTCAGCCTCGGCGCCGCTCGCGCGGGTGAGCCCGGCAAGCATCGGCC
CGCGGTGGTCGTTTCCGTGGACGAGCTACTCACCGGAATCGACGACGAACTCGTTGTCGTCGTGCCGGTGTCAAGCTCGC
GCTCCCGCACCCCACTCCGGCCACCTGTCGCGCCCTCAGAAGGTGTAGCTGCCGATAGCGTCGCGGTGTGCCGCGGCGTC
CGCGCGGTCGCTCGTGCCCGACTCGTGGAGCGACTCGGCGCCCTCAAACCCGCCACGATGCGCGCAATCGAAAACGCCCT
GACCCTGATCCTCGGCCTCCCGACGGGACCTGAGCGCGGCGAGGCGGCGACCCATTCTCCCGTACGGTGGACGGGTGGCC
GGGACCCGTGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 12094.74 Da
• Isoelectric Point: 7.0370

>AT163755 WP_003031347.1 NZ_CP056595:3836276-3836593 [Citrobacter freundii]
MSKITWGLQRDITPRLGARLVQEGNRLHYLADRASMTGKFSDIEYRKLDETFPHFIRQMESMLTTGELSTHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 318 bp

>AT163755 NZ_CP072762:2323762-2323995 [Mycobacterium tuberculosis]
ATGTCTACATCCACGACGATTAGGGTTTCAACCCAGACTCGGGATCGTCTGGCCGCCCAAGCCCGCGAACGGGGAATCTC
GATGTCGGCTCTGCTCACCGAACTGGCCGCCCAGGCCGAGCGCCAGGCAATCTTCCGCGCCGAACGCGAGGCCTCGCACG
CCGAGACGACCACCCAGGCAGTCCGCGACGAGGACCGCGAGTGGGAGGGCACGGTAGGCGACGGCCTTGGCTGA

Structures

Toxin

• AlphaFold DB: A0A1B7JLK7

Antitoxin

• AlphaFold DB: A0A1B7JLJ2