Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3601351..3601971 Replicon chromosome
Accession NZ_CP056595
Organism Citrobacter freundii strain RHBSTW-00342

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag HV167_RS17360 Protein ID WP_002892050.1
Coordinates 3601753..3601971 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID R8WZW8
Locus tag HV167_RS17355 Protein ID WP_003021733.1
Coordinates 3601351..3601725 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HV167_RS17345 3596497..3597690 + 1194 WP_003835922.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
HV167_RS17350 3597713..3600862 + 3150 WP_003021736.1 multidrug efflux RND transporter permease subunit -
HV167_RS17355 3601351..3601725 + 375 WP_003021733.1 Hha toxicity modulator TomB Antitoxin
HV167_RS17360 3601753..3601971 + 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
HV167_RS17365 3602278..3602829 + 552 WP_003835924.1 maltose O-acetyltransferase -
HV167_RS17370 3602946..3603416 + 471 WP_003021724.1 YlaC family protein -
HV167_RS17375 3603495..3603635 - 141 WP_005125190.1 type B 50S ribosomal protein L36 -
HV167_RS17380 3603637..3603897 - 261 WP_003021719.1 type B 50S ribosomal protein L31 -
HV167_RS17385 3604086..3605639 + 1554 WP_071696870.1 EAL domain-containing protein -
HV167_RS17390 3605691..3606044 - 354 WP_071696871.1 DUF1428 family protein -
HV167_RS17395 3606109..3606738 - 630 WP_003021707.1 membrane protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T163754 WP_002892050.1 NZ_CP056595:3601753-3601971 [Citrobacter freundii]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T163754 NZ_CP072762:2261204-2261602 [Mycobacterium tuberculosis]
ATGATCGTCGACACCTCGGTCTGGATCGCATATCTCTCCACGTCAGAGTCGTTGGCCAGTCGCTGGCTAGCCGATCGCAT
TGCCGCTGACTCGACGGTGATCGTGCCCGAGGTGGTGATGATGGAGCTGCTGATCGGTAAGACCGATGAGGACACCGCCG
CACTGCGCCGACGGCTCCTGCAGCGATTCGCTATCGAACCGCTGGCCCCGGTCCGCGACGCGGAAGATGCCGCCGCCATT
CACCGGCGCTGTCGTCGCGGCGGCGACACCGTACGCAGCCTGATCGATTGCCAGGTGGCCGCGATGGCGTTGCGGATCGG
GGTCGCCGTGGCGCATCGTGATCGCGACTACGAGGCGATCCGCACACATTGCGGACTACGCACCGAGCCGTTGTTCTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14427.23 Da        Isoelectric Point: 5.5653

>AT163754 WP_003021733.1 NZ_CP056595:3601351-3601725 [Citrobacter freundii]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEQSNHGWVNDPTSATSLQLNELIEHIATFALNYKIKYNEDNKLIAQIDEYL
DDTFMLFSSYGINTQDLQKWRKSGNRLFRCFVNATRANPVSLSC

Download         Length: 375 bp

>AT163754 NZ_CP072762:2260976-2261203 [Mycobacterium tuberculosis]
GTGTCACGGACCAACATCGAGATCGACGACGAACTCGTGGCCGCCGCACAGCGGATGTACCGACTCGATTCCAAGCGAAG
TGCCGTCGACCTCGCGCTGCGCCGGCTCGTGGGTGAACCGTTGGGCCGCGATGAGGCTTTGGCGCTGCAGGGCAGCGGTT
TCGACTTCAGCAACGATGAGATCGAATCGTTCTCGGATACGGACCGCAAGCTCGCCGACGAGTCGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB R8WZW8

References