Detailed information of TA system TA163753

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2416720..2417359
• Replicon: chromosome
• Accession: NZ_CP056595
• Organism: Citrobacter freundii strain RHBSTW-00342

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A8B5QF36
• Locus tag: HV167_RS11550
• Protein ID: WP_003020221.1
• Coordinates: 2416720..2416896 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: HV167_RS11555
• Protein ID: WP_128298791.1
• Coordinates: 2416943..2417359 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV167_RS11530	2413498..2413728	-	231	WP_003020233.1	DUF2554 family protein	-
HV167_RS11535	2413918..2414043	-	126	WP_003020230.1	DUF2474 domain-containing protein	-
HV167_RS11540	2414043..2415053	-	1011	WP_003020227.1	cytochrome d ubiquinol oxidase subunit II	-
HV167_RS11545	2415053..2416456	-	1404	WP_003020224.1	cytochrome ubiquinol oxidase subunit I	-
HV167_RS11550	2416720..2416896	+	177	WP_003020221.1	type II toxin-antitoxin system HicA family toxin	Toxin
HV167_RS11555	2416943..2417359	+	417	WP_128298791.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
HV167_RS11560	2417452..2418861	+	1410	WP_071696130.1	PLP-dependent aminotransferase family protein	-
HV167_RS11565	2419189..2420334	+	1146	WP_061548295.1	ABC transporter substrate-binding protein	-
HV167_RS11570	2420351..2421367	+	1017	WP_071696128.1	ABC transporter ATP-binding protein	-
HV167_RS11575	2421368..2422312	+	945	WP_181558349.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-65)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6769.80 Da
• Isoelectric Point: 11.2510

>T163753 WP_003020221.1 NZ_CP056595:2416720-2416896 [Citrobacter freundii]
VKQSEFRRWLESQGVAVTNGSNHLKLRYQGRRSVMPRHPGDEIKEALRKAIIKQLDLQ

Nucleotide

• Length: 177 bp

>T163753 NZ_CP072762:c2237580-2237236 [Mycobacterium tuberculosis]
GTGGTGATTAGTCGTGCCGAGATCTACTGGGCTGACCTCGGGCCGCCATCAGGCAGTCAGCCGGCGAAGCGCCGCCCGGT
GCTCGTAATCCAGTCAGATCCGTACAACGCAAGTCGCCTTGCCACTGTGATCGCAGCGGTGATCACGTCCAATACGGCGC
TGGCGGCAATGCCCGGCAACGTGTTCTTGCCCGCGACCACAACGCGACTGCCACGTGACTCGGTCGTCAACGTCACGGCG
ATTGTCACGCTCAACAAGACTGACCTCACCGACCGAGTTGGGGAGGTGCCAGCGAGCTTGATGCACGAGGTTGACCGAGG
ACTTCGTCGCGTACTGGACCTTTGA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15154.42 Da
• Isoelectric Point: 4.7238

>AT163753 WP_128298791.1 NZ_CP056595:2416943-2417359 [Citrobacter freundii]
MRYPVTLTPAVEGGFVVSFPDIPEALTQGNTRHDALQAARAALITAFEFYFDDNEAIPLPSAVSAEDDYVEIPLSVASKV
LLLNAFLESKITQQELANRIGRPKQEITRLFDLKHTTKIDAVQIAARALGKELALTML

Nucleotide

• Length: 417 bp

>AT163753 NZ_CP072762:c2237822-2237574 [Mycobacterium tuberculosis]
ATGAAGACAGCTATTTCTCTGCCGGATGAGACGTTCGATCGGGTATCGCGGCGTGCGAGTGAGCTCGGCATGAGTCGGTC
CGAGTTCTTCACGAAGGCTGCGCAGCGCTACCTGCACGAGCTGGACGCCCAATTGCTCACGGGCCAGATCGACAGGGCTC
TAGAGAGCATCCATGGCACCGACGAAGCGGAGGCCCTCGCCGTGGCCAACGCATACCGCGTGCTAGAAACCATGGACGAT
GAGTGGTGA

Structures

Toxin

• AlphaFold DB: A0A8B5QF36