Detailed information of TA system TA163743

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yfjZ/CbtA-CbeA
• Location: 752056..752735
• Replicon: chromosome
• Accession: NZ_CP056595
• Organism: Citrobacter freundii strain RHBSTW-00342

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: A0A7D6Z078
• Locus tag: HV167_RS03675
• Protein ID: WP_048215514.1
• Coordinates: 752394..752735 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: A0A0F6RHY7
• Locus tag: HV167_RS03670
• Protein ID: WP_046495841.1
• Coordinates: 752056..752373 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV167_RS03630	747615..748448	+	834	WP_103856759.1	DUF945 domain-containing protein	-
HV167_RS03635	748650..749354	+	705	WP_046495813.1	WYL domain-containing protein	-
HV167_RS03640	749351..749965	+	615	WP_046495819.1	hypothetical protein	-
HV167_RS03645	750054..750464	+	411	WP_046495825.1	hypothetical protein	-
HV167_RS03650	750542..750778	+	237	WP_046495830.1	DUF905 domain-containing protein	-
HV167_RS03655	750857..751315	+	459	WP_046495834.1	antirestriction protein	-
HV167_RS03660	751331..751807	+	477	WP_046495838.1	RadC family protein	-
HV167_RS03665	751816..752037	+	222	WP_000691995.1	DUF987 domain-containing protein	-
HV167_RS03670	752056..752373	+	318	WP_046495841.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
HV167_RS03675	752394..752735	+	342	WP_048215514.1	TA system toxin CbtA family protein	Toxin
HV167_RS03680	752851..753684	+	834	WP_048215515.1	DUF4942 domain-containing protein	-
HV167_RS03690	753988..754494	+	507	WP_071696310.1	G/U mismatch-specific DNA glycosylase	-
HV167_RS03695	754525..756522	-	1998	WP_071696309.1	TonB-dependent receptor	-
HV167_RS03700	756533..757582	-	1050	WP_071696308.1	YncE family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	710225..761580	51355

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (2-112)

Antitoxin

• Antitoxin CbeA (5-104)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 13014.97 Da
• Isoelectric Point: 10.4182

>T163743 WP_048215514.1 NZ_CP056595:752394-752735 [Citrobacter freundii]
MKPQSAATSRAVKPCLSPVAIWQILLTRLLEQHYGLTLNDTPFRDESVIQEHIDAGITLANAVNFLVEKYELVRIDRRRF
SWQEQSPYLRAADILRARQATGLLRQSRNNVVR

Nucleotide

• Length: 342 bp

>T163743 NZ_CP072762:c1575877-1575476 [Mycobacterium tuberculosis]
ATGATCCTTGTCGACTCCGATGTGCTGATCGCGCATTTGCGGGGTGTCGTTGCTGCTCGCGATTGGCTTGTCAGCGCCCG
CAAGGACGGACCGCTGGCGATCAGCGTGGTGTCCACCGCCGAACTCATCGGCGGAATGCGGACCGCCGAACGGCGCGAGG
TGTGGCGCCTGCTTGCATCGTTTCGGGTACAGCCAGCAACCGAGGTAATCGCACGCCGCGCCGGCGACATGATGCGCCGA
TATCGTCGCAGCCACAACCGGATTGGACTTGGCGACTATCTGATAGCTGCTACCGCCGACGTCCAGGACCTGCAATTGGC
AACCCTCAACGTGTGGCATTTCCCCATGTTCGAGCAGCTGAAACCACCATTTGCGGTGCCGGGGCACCGACCGCGGGCAT
GA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11869.49 Da
• Isoelectric Point: 6.6245

>AT163743 WP_046495841.1 NZ_CP056595:752056-752373 [Citrobacter freundii]
MSNITWGLQRDITPRLGTRLVQEGNRLHYLADRASITGKFSDAECQKLDETFPHFISQMESMLTSGELNPLHVHCVTLYH
TGFTCEADTLGSCGYVYIAIYPTKR

Nucleotide

• Length: 318 bp

>AT163743 NZ_CP072762:c1576131-1575874 [Mycobacterium tuberculosis]
ATGAAGCGGACCAACATCTACCTCGACGAGGAGCAGACGGCAAGCCTCGACAAGTTGGCCGCGCAAGAAGGTGTTTCGCG
CGCCGAGCTGATCCGCCTCCTGCTGAACCGAGCCCTCACCACCGCTGGGGACGACCTTGCATCGGACCTGCAGGCTATAA
ACGATTCGTTCGGCACGCTTCGCCACCTGGATCCGCCGGTGCGTCGCTCCGGTGGTCGTGAACAGCACCTTGCCCAGGTG
TGGCGCGCCACCTCATGA

Structures

Toxin

• AlphaFold DB: A0A7D6Z078

Antitoxin

• AlphaFold DB: A0A0F6RHY7