Detailed information of TA system TA163730

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2548749..2549388
• Replicon: chromosome
• Accession: NZ_CP056592
• Organism: Citrobacter freundii strain RHBSTW-00350

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A8B5QF36
• Locus tag: HV171_RS12325
• Protein ID: WP_003020221.1
• Coordinates: 2549212..2549388 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: HV171_RS12320
• Protein ID: WP_128298791.1
• Coordinates: 2548749..2549165 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV171_RS12300	2543796..2544740	-	945	WP_003843727.1	ABC transporter permease	-
HV171_RS12305	2544741..2545757	-	1017	WP_071696128.1	ABC transporter ATP-binding protein	-
HV171_RS12310	2545774..2546919	-	1146	WP_061548295.1	ABC transporter substrate-binding protein	-
HV171_RS12315	2547247..2548656	-	1410	WP_071696130.1	PLP-dependent aminotransferase family protein	-
HV171_RS12320	2548749..2549165	-	417	WP_128298791.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
HV171_RS12325	2549212..2549388	-	177	WP_003020221.1	type II toxin-antitoxin system HicA family toxin	Toxin
HV171_RS12330	2549652..2551055	+	1404	WP_003020224.1	cytochrome ubiquinol oxidase subunit I	-
HV171_RS12335	2551055..2552065	+	1011	WP_003020227.1	cytochrome d ubiquinol oxidase subunit II	-
HV171_RS12340	2552065..2552190	+	126	WP_003020230.1	DUF2474 domain-containing protein	-
HV171_RS12345	2552380..2552610	+	231	WP_003020233.1	DUF2554 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-65)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6769.80 Da
• Isoelectric Point: 11.2510

>T163730 WP_003020221.1 NZ_CP056592:c2549388-2549212 [Citrobacter freundii]
VKQSEFRRWLESQGVAVTNGSNHLKLRYQGRRSVMPRHPGDEIKEALRKAIIKQLDLQ

Nucleotide

• Length: 177 bp

>T163730 NZ_CP072762:718285-718692 [Mycobacterium tuberculosis]
GTGAGCACGACGCCGGCCGCCGGAGTGCTCGACACGTCGGTGTTCATCGCGACCGAAAGCGGCCGGCAACTCGACGAGGC
GCTGATCCCCGACCGGGTCGCCACCACCGTCGTCACCCTCGCCGAACTGCGCGTCGGCGTGCTGGCCGCGGCGACGACCG
ACATCCGGGCTCAACGCCTGGCGACCCTGGAATCCGTTGCCGATATGGAAACGTTGCCCGTCGACGACGATGCCGCCCGA
ATGTGGGCCCGATTGCGGATCCATCTTGCCGAGTCCGGTCGCCGGGTGCGGATCAACGACCTGTGGATCGCGGCCGTCGC
GGCATCGCGAGCGCTGCCGGTCATCACCCAGGACGACGACTTCGCCGCCCTCGACGGTGCGGCCAGTGTGGAGATCATTC
GGGTCTGA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15154.42 Da
• Isoelectric Point: 4.7238

>AT163730 WP_128298791.1 NZ_CP056592:c2549165-2548749 [Citrobacter freundii]
MRYPVTLTPAVEGGFVVSFPDIPEALTQGNTRHDALQAARAALITAFEFYFDDNEAIPLPSAVSAEDDYVEIPLSVASKV
LLLNAFLESKITQQELANRIGRPKQEITRLFDLKHTTKIDAVQIAARALGKELALTML

Nucleotide

• Length: 417 bp

>AT163730 NZ_CP072762:718028-718288 [Mycobacterium tuberculosis]
ATGTCTGAGGTGGCCTCGCGTGAGCTGCGTAACGATACGGCCGGCGTGCTGCGCCGCGTGCGGGCAGGGGAGGACGTCAC
CATCACCGTCAGCGGCCGTCCGGTCGCGGTGCTTACCCCGGTTCGTCCGCGGCGCCGGCGTTGGCTGAGCAAAACGGAGT
TCCTGTCGCGGTTGCGCGGCGCTCAAGCCGATCCCGGGCTCCGTAACGACCTCGCGGTCCTTGCCGGCGACACGACCGAG
GATCTCGGGCCGATCCGGTGA

Structures

Toxin

• AlphaFold DB: A0A8B5QF36