Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1335526..1336146 Replicon chromosome
Accession NZ_CP056592
Organism Citrobacter freundii strain RHBSTW-00350

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag HV171_RS06415 Protein ID WP_002892050.1
Coordinates 1335526..1335744 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID R8WZW8
Locus tag HV171_RS06420 Protein ID WP_003021733.1
Coordinates 1335772..1336146 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HV171_RS06380 1330759..1331388 + 630 WP_003021707.1 membrane protein -
HV171_RS06385 1331453..1331806 + 354 WP_071696871.1 DUF1428 family protein -
HV171_RS06390 1331858..1333411 - 1554 WP_071696870.1 EAL domain-containing protein -
HV171_RS06395 1333600..1333860 + 261 WP_003021719.1 type B 50S ribosomal protein L31 -
HV171_RS06400 1333862..1334002 + 141 WP_005125190.1 type B 50S ribosomal protein L36 -
HV171_RS06405 1334081..1334551 - 471 WP_003021724.1 YlaC family protein -
HV171_RS06410 1334668..1335219 - 552 WP_003835924.1 maltose O-acetyltransferase -
HV171_RS06415 1335526..1335744 - 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
HV171_RS06420 1335772..1336146 - 375 WP_003021733.1 Hha toxicity modulator TomB Antitoxin
HV171_RS06425 1336635..1339784 - 3150 WP_003021736.1 multidrug efflux RND transporter permease subunit -
HV171_RS06430 1339807..1341000 - 1194 WP_003835922.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T163729 WP_002892050.1 NZ_CP056592:c1335744-1335526 [Citrobacter freundii]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T163729 NZ_CP072762:716667-717062 [Mycobacterium tuberculosis]
ATGGTGATCGACACGTCCGCGCTCGTTGCGATGCTCAGCGACGAGCCAGACGCAGAGCGGTTCGAGGCCGCCGTCGAAGC
CGACCACATCCGGCTGATGTCGACGGCGTCTTACCTGGAAACGGCACTCGTGATAGAAGCCCGCTTCGGTGAACCGGGCG
GACGTGAGCTGGATCTGTGGCTTCATCGCGCCGCGGTCGACCTTGTTGCCGTGCATGCCGACCAAGCGGATGCCGCGCGC
GCCGCCTACCGCACGTACGGCAAGGGAAGGCATCGTGCGGGGCTCAACTACGGCGACTGCTTCTCATACGGCCTCGCCAA
GATCAGCGGCCAGCCACTCCTGTTCAAGGGCGAAGATTTCCAACACACCGACATCGCCACGGTCGCGCTGCCCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14427.23 Da        Isoelectric Point: 5.5653

>AT163729 WP_003021733.1 NZ_CP056592:c1336146-1335772 [Citrobacter freundii]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEQSNHGWVNDPTSATSLQLNELIEHIATFALNYKIKYNEDNKLIAQIDEYL
DDTFMLFSSYGINTQDLQKWRKSGNRLFRCFVNATRANPVSLSC

Download         Length: 375 bp

>AT163729 NZ_CP072762:716413-716667 [Mycobacterium tuberculosis]
ATGGCGCTGAGTATCAAGCACCCGGAAGCCGACCGGCTCGCGCGAGCGCTTGCGGCGCGCACCGGCGAGACGTTGACCGA
GGCAGTGGTTACCGCGTTGCGCGAGCGGCTCGCTCGTGAGACTGGGCGTGCCCGTGTTGTCCCGTTGCGCGACGAGCTTG
CCGCGATTCGGCACCGGTGCGCAGCGTTGCCGGTGGTCGACAACCGGTCCGCTGAGGCGATTCTCGGCTATGACGAGCGC
GGATTGCCGGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB R8WZW8

References