Detailed information of TA system TA163723

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 135464..136107
• Replicon: plasmid pRHBSTW-00370_3
• Accession: NZ_CP056575
• Organism: Citrobacter freundii strain RHBSTW-00370

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: A0A6C0NGR2
• Locus tag: HV178_RS26865
• Protein ID: WP_008322222.1
• Coordinates: 135464..135880 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: A0A6C0NE67
• Locus tag: HV178_RS26870
• Protein ID: WP_008322219.1
• Coordinates: 135877..136107 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV178_RS26840	131562..131731	-	170	Protein_139	DUF596 domain-containing protein	-
HV178_RS26845	131925..132341	-	417	WP_008322233.1	type II toxin-antitoxin system VapC family toxin	-
HV178_RS26850	132338..132568	-	231	WP_001261276.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
HV178_RS26855	132821..133843	-	1023	WP_008322227.1	helicase UvrD	-
HV178_RS26860	133828..135390	-	1563	WP_008322224.1	AAA family ATPase	-
HV178_RS26865	135464..135880	-	417	WP_008322222.1	type II toxin-antitoxin system VapC family toxin	Toxin
HV178_RS26870	135877..136107	-	231	WP_008322219.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
HV178_RS26875	136877..137095	+	219	WP_032934863.1	type II toxin-antitoxin system antitoxin CcdA	-
HV178_RS26880	137097..137402	+	306	WP_008322213.1	type II toxin-antitoxin system toxin CcdB	-
HV178_RS26885	137404..137718	+	315	WP_008322211.1	hypothetical protein	-
HV178_RS26890	137708..138499	+	792	WP_008322208.1	site-specific integrase	-
HV178_RS26895	138698..140065	-	1368	WP_008322207.1	GntP family transporter	-
HV178_RS26900	140151..140927	-	777	WP_008322204.1	HPr family phosphocarrier protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..148724	148724

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-50)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15188.56 Da
• Isoelectric Point: 7.1361

>T163723 WP_008322222.1 NZ_CP056575:c135880-135464 [Citrobacter freundii]
VNKTYMLDTNICSFIMREQPEAVLKRLEQAVLRNQRIVVSAITYSEMRFGATGPKASPRHIELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Nucleotide

• Length: 417 bp

>T163723 NZ_CP072762:c640644-640231 [Mycobacterium tuberculosis]
GTGAGAGCATCGCCCACTTCGCCGCCGGAGCAGGTGGTCGTCGACGCGAGTGCCATGGTGGATCTACTGGCTCGCACTAG
CGATCGGTGCTCTGCGGTGCGCGCGCGGCTGGCTCGGACCGCGATGCACGCGCCGGCGCACTTCGATGCAGAGGTGTTGT
CGGCGCTGGGGCGCATGCAGCGCGCCGGCGCACTCACCGTTGCCTATGTCGATGCGGCACTGGAGGAGTTGCGACAGGTG
CCGGTGACTCGACACGGTCTTTCGTCGCTGCTTGCTGGAGCGTGGTCGCGCCGCGACACCCTCCGCCTGACCGATGCCCT
CTACGTCGAGCTGGCCGAAACGGCAGGTCTGGTGTTGTTGACCACCGACGAAAGATTGGCACGCGCCTGGCCCTCGGCTC
ACGCCATCGGCTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8736.84 Da
• Isoelectric Point: 4.4692

>AT163723 WP_008322219.1 NZ_CP056575:c136107-135877 [Citrobacter freundii]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQLEKADPDFMAEREDVVTDEGRFNL

Nucleotide

• Length: 231 bp

>AT163723 NZ_CP072762:c640907-640641 [Mycobacterium tuberculosis]
TTGTTGAGCCGGCGCACGAAAACGATCGTTGTGTGTACATTGGTGTGTATGGCTCGGTTGAACGTGTATGTGCCCGACGA
ATTGGCGGAGCGCGCCAGGGCGCGGGGCTTGAACGTCTCGGCGCTGACTCAGGCCGCGATCAGTGCCGAGTTGGAGAACT
CCGCAACCGATGCGTGGCTTGAGGGGTTGGAACCCAGAAGCACCGGCGCTCGGCATGATGACGTGCTGGGTGCGATCGAT
GCCGCTCGCGATGAGTTCGAAGCGTGA

Structures

Toxin

• AlphaFold DB: A0A6C0NGR2

Antitoxin

• AlphaFold DB: A0A6C0NE67