Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /RES-TIGR02293
Location 20040..20950 Replicon plasmid pRHBSTW-00373_2
Accession NZ_CP056568
Organism Klebsiella oxytoca strain RHBSTW-00373

Toxin (Protein)


Gene name - Uniprot ID R4YBC5
Locus tag HV180_RS28835 Protein ID WP_004144067.1
Coordinates 20480..20950 (+) Length 157 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID A0A6A8PHD0
Locus tag HV180_RS28830 Protein ID WP_023287184.1
Coordinates 20040..20483 (+) Length 148 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HV180_RS28810 15451..17226 + 1776 Protein_15 ThiF family adenylyltransferase -
HV180_RS28815 17283..18251 - 969 WP_000654811.1 IS5 family transposase -
HV180_RS28820 18289..18744 + 456 Protein_17 Mov34/MPN/PAD-1 family protein -
HV180_RS28825 18791..19939 + 1149 Protein_18 translesion error-prone DNA polymerase V subunit UmuC -
HV180_RS28830 20040..20483 + 444 WP_023287184.1 DUF2384 domain-containing protein Antitoxin
HV180_RS28835 20480..20950 + 471 WP_004144067.1 RES family NAD+ phosphorylase Toxin
HV180_RS28840 21037..22032 + 996 WP_077254794.1 IS5 family transposase -
HV180_RS28845 22034..22216 + 183 Protein_22 IS5/IS1182 family transposase -
HV180_RS28850 22286..23448 + 1163 WP_088941748.1 IS3 family transposase -
HV180_RS28855 23608..23784 - 177 Protein_24 transposase -
HV180_RS28860 23870..24334 - 465 Protein_25 transposase -
HV180_RS28865 24386..25306 - 921 WP_004181990.1 response regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - pla 1..202470 202470
- inside IScluster/Tn - - 17283..24244 6961


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

RES domain

(3-148)

Antitoxin

Antitoxin Xre-like, helix-turn-helix domain

(30-89)


Sequences

Toxin        


Download         Length: 157 a.a.        Molecular weight: 17573.83 Da        Isoelectric Point: 4.5152

>T163700 WP_004144067.1 NZ_CP056568:20480-20950 [Klebsiella oxytoca]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPDNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Download         Length: 471 bp

>T163700 NZ_CP072761:c2903133-2902714 [Mycobacterium tuberculosis]
GTGACGGCACTGCTCGATGTCAATGTGCTGATCGCGCTGGGCTGGCCGAATCACGTTCACCATGCGGCCGCGCAGCGATG
GTTCACGCAGTTCTCCTCGAATGGGTGGGCCACCACGCCGATCACCGAGGCAGGGTATGTCCGAATTTCAAGCAATCGCA
GTGTGATGCAGGTGTCGACCACGCCGGCTATCGCGATCGCTCAGTTGGCGGCGATGACTTCTCTTGCCGGGCACACGTTT
TGGCCTGACGATGTGCCACTGATCGTTGGGAGCGCCGGCGATCGCGATGCGGTGTCCAACCACCGTCGGGTCACCGACTG
CCATCTCATCGCCTTGGCCGCGCGCTACGGGGGCCGGTTGGTCACATTCGATGCCGCACTGGCCGATTCAGCATCCGCAG
GCCTCGTCGAGGTGTTGTAG

Antitoxin


Download         Length: 148 a.a.        Molecular weight: 16522.84 Da        Isoelectric Point: 10.3013

>AT163700 WP_023287184.1 NZ_CP056568:20040-20483 [Klebsiella oxytoca]
MKTFSLSSSPARPQRLWQVAGLNNADGVALLGQINEGLDGKVVNRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDREAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Download         Length: 444 bp

>AT163700 NZ_CP072761:c2903354-2903130 [Mycobacterium tuberculosis]
ATGCGCACCACGTTGCAGATTGATGATGATGTTCTAGAAGATGCTCGTAGCATCGCGCGGTCGGAGGGCAAGTCAGTCGG
CGCGGTAATTTCTGAGTTGGCGCGTAGGTCGCTCCGTCCGGTTGGGATTGTCGAGGTTGACGGATTTCCGGTTTTTGATG
TTCCGCCGGATGCGCCGACGGTGACTTCCGAGGATGTCGTCCGCGCGCTCGAGGACGACGTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A384IED8

Antitoxin

Source ID Structure
AlphaFold DB A0A6A8PHD0

References