Detailed information of TA system TA163698

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 4512184..4512803
• Replicon: chromosome
• Accession: NZ_CP056567
• Organism: Klebsiella oxytoca strain RHBSTW-00373

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: H3N9D8
• Locus tag: HV180_RS21420
• Protein ID: WP_004099646.1
• Coordinates: 4512585..4512803 (+)
• Length: 73 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: -
• Locus tag: HV180_RS21415
• Protein ID: WP_004099648.1
• Coordinates: 4512184..4512558 (+)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV180_RS21405	4507340..4508533	+	1194	WP_004111040.1	multidrug efflux RND transporter periplasmic adaptor subunit AcrA	-
HV180_RS21410	4508556..4511702	+	3147	WP_004099650.1	multidrug efflux RND transporter permease subunit	-
HV180_RS21415	4512184..4512558	+	375	WP_004099648.1	Hha toxicity modulator TomB	Antitoxin
HV180_RS21420	4512585..4512803	+	219	WP_004099646.1	hemolysin expression modulator Hha	Toxin
HV180_RS21425	4512964..4513530	+	567	WP_142447582.1	maltose O-acetyltransferase	-
HV180_RS21430	4513667..4514137	+	471	WP_004111038.1	YlaC family protein	-
HV180_RS21435	4514112..4515566	-	1455	WP_017146481.1	PLP-dependent aminotransferase family protein	-
HV180_RS21440	4515669..4516367	+	699	WP_004099639.1	GNAT family N-acetyltransferase	-
HV180_RS21445	4516364..4516504	-	141	WP_003859006.1	type B 50S ribosomal protein L36	-
HV180_RS21450	4516504..4516767	-	264	WP_004099638.1	type B 50S ribosomal protein L31	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (13-69)

Antitoxin

• Biofilm formation regulator YbaJ (1-116)

Sequences

Toxin

Protein

• Length: 73 a.a.
• Molecular weight: 8640.05 Da
• Isoelectric Point: 8.9008

>T163698 WP_004099646.1 NZ_CP056567:4512585-4512803 [Klebsiella oxytoca]
MSDKTLTKIDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPPSVWKFIR

Nucleotide

• Length: 219 bp

>T163698 NZ_CP072761:c2405441-2405124 [Mycobacterium tuberculosis]
ATGACGCGCAGGCTGCGCGTCCATAACGGGGTTGAAGACGACCTATTCGAGGCGTTTTCCTACTACGCGGACGCGGCGCC
AGATCAGATCGATCGGCTTTACAACTTGTTTGTCGATGCCGTGACGAAGCGGATTCCGCAGGCTCCGAACGCGTTTGCGC
CGTTATTCAAGCACTATCGACACATCTACCTCCGGCCCTTCAGGTACTACGTTGCCTATCGGACGACCGACGAGGCTATC
GACATACTGGCTGTTCGGCACGGAATGGAGAACCCGAACGCGGTCGAGGCTGAGATCTCTGGCCGCACCTTCGAGTGA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 14350.09 Da
• Isoelectric Point: 4.8989

>AT163698 WP_004099648.1 NZ_CP056567:4512184-4512558 [Klebsiella oxytoca]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIAAFALNYKIKYAEDNKLVTQLDEYL
DDTFVLFSNYGINTADLQKWRKSGNRLFRCFVNASRENPASLSC

Nucleotide

• Length: 375 bp

>AT163698 NZ_CP072761:c2405653-2405438 [Mycobacterium tuberculosis]
GTGGTGGTCAACCGGGCATTGCTGGCGAGCGTCGACGCACTGTCGCGTGATGAGCAGATTGAGCTCGTCGAGCACATCAA
CGGAAACCTAGCCGAGGGCATGCATATCAGCGAGGCCAACCAGGCGCTCATCGAAGCGCGGGCCAATGACACCGACGATG
CTCATTGGTCCACCATTGATGACTTCGACAAGCGGATCCGCGCCCGGCTCGGATGA

Structures

Toxin

• AlphaFold DB: A0A0H3H713