Detailed information of TA system TA163685

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 4360502..4361037
• Replicon: chromosome
• Accession: NZ_CP056559
• Organism: Citrobacter freundii strain RHBSTW-00408

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: HV186_RS20710
• Protein ID: WP_137399199.1
• Coordinates: 4360750..4361037 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: D4BKM9
• Locus tag: HV186_RS20705
• Protein ID: WP_006688247.1
• Coordinates: 4360502..4360753 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV186_RS20675	4355514..4356362	+	849	WP_135912711.1	alpha-D-ribose 1-methylphosphonate 5-phosphate C-P-lyase PhnJ	-
HV186_RS20680	4356359..4357117	+	759	WP_115259672.1	phosphonate C-P lyase system protein PhnK	-
HV186_RS20685	4357226..4357906	+	681	WP_117343579.1	phosphonate C-P lyase system protein PhnL	-
HV186_RS20690	4357903..4359039	+	1137	WP_096758663.1	alpha-D-ribose 1-methylphosphonate 5-triphosphate diphosphatase	-
HV186_RS20695	4359042..4359587	+	546	WP_115259675.1	ribose 1,5-bisphosphokinase	-
HV186_RS20700	4359660..4360418	+	759	WP_181490714.1	phosphonate metabolism protein PhnP	-
HV186_RS20705	4360502..4360753	+	252	WP_006688247.1	plasmid stabilization protein	Antitoxin
HV186_RS20710	4360750..4361037	+	288	WP_137399199.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HV186_RS20715	4361034..4361363	-	330	WP_096758667.1	hypothetical protein	-
HV186_RS20720	4361432..4363696	-	2265	WP_181490715.1	hybrid sensor histidine kinase/response regulator	-
HV186_RS20725	4363811..4365334	+	1524	WP_181490716.1	sugar ABC transporter ATP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-86)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11173.02 Da
• Isoelectric Point: 10.2830

>T163685 WP_137399199.1 NZ_CP056559:4360750-4361037 [Citrobacter freundii]
MSYTVKFREDALKEWQKLDKTIQQQFAKELKKCGENPHVPSAKLRGIKDCYKIQLRTSRFRLVYQVIDDTLVIAVVAVGK
RERSEVYNLASERLR

Nucleotide

• Length: 288 bp

>T163685 NZ_CP072761:1688141-1688458 [Mycobacterium tuberculosis]
GTGAACGCGCCGTTGCGTGGTCAGGTCTATCGATGCGACCTCGGATACGGGGCCAAACCGTGGCTCATCGTCTCCAACAA
CGCCCGCAACCGTCACACCGCCGACGTGGTGGCTGTGCGCCTGACAACAACGCGGAGAACCATACCGACCTGGGTCGCCA
TGGGCCCCAGCGATCCATTGACCGGATACGTCAACGCGGACAACATCGAGACCCTCGGCAAAGACGAGCTCGGTGACTAC
CTCGGTGAGGTCACGCCGGCGACGATGAACAAAATCAACACGGCGCTCGCGACCGCGCTGGGGCTACCGTGGCCATGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9215.53 Da
• Isoelectric Point: 4.2965

>AT163685 WP_006688247.1 NZ_CP056559:4360502-4360753 [Citrobacter freundii]
MPHIILSDTTASVSELKKNPMATVSAGDGFPVAILNRNQPAFYCVPAELYEKMLDALDDQQLVKLVHERSQQPLVDVDLD
SYL

Nucleotide

• Length: 252 bp

>AT163685 NZ_CP072761:1687842-1688144 [Mycobacterium tuberculosis]
GTGCCGTTTCTGGTTGCATTATCCGGTATCATCTCGGGCGTGCGTGATCATTCGATGACCGTGCGGCTCGACCAGCAAAC
TCGCCAGCGCCTGCAAGACATTGTGAAAGGCGGATACCGGAGCGCTAATGCGGCGATCGTCGACGCCATCAACAAGCGCT
GGGAGGCGCTACACGATGAGCAACTCGACGCCGCCTACGCGGCCGCGATCCATGACAATCCGGCGTACCCGTACGAGTCT
GAGGCCGAACGGAGCGCCGCGCGGGCCCGGCGCAACGCCAGGCAGCAGCGCTCGGCACAGTGA

Structures

Antitoxin

• AlphaFold DB: A0A7X1BT32