Detailed information of TA system TA163684

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 4178854..4179370
• Replicon: chromosome
• Accession: NZ_CP056559
• Organism: Citrobacter freundii strain RHBSTW-00408

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A5I6CHB9
• Locus tag: HV186_RS19840
• Protein ID: WP_000220581.1
• Coordinates: 4178854..4179138 (-)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: HV186_RS19845
• Protein ID: WP_115259576.1
• Coordinates: 4179128..4179370 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV186_RS19825	4174055..4175707	+	1653	WP_181490669.1	alpha,alpha-phosphotrehalase	-
HV186_RS19830	4176126..4178264	+	2139	WP_115259574.1	anaerobic ribonucleoside-triphosphate reductase	-
HV186_RS19835	4178386..4178850	+	465	WP_096759476.1	anaerobic ribonucleoside-triphosphate reductase-activating protein	-
HV186_RS19840	4178854..4179138	-	285	WP_000220581.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HV186_RS19845	4179128..4179370	-	243	WP_115259576.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
HV186_RS19850	4179448..4181361	-	1914	WP_181490670.1	BglG family transcription antiterminator	-
HV186_RS19855	4181383..4182123	-	741	WP_146717606.1	KDGP aldolase family protein	-
HV186_RS19860	4182120..4183238	-	1119	WP_146717607.1	DgaE family pyridoxal phosphate-dependent ammonia lyase	-
HV186_RS19865	4183222..4184355	-	1134	WP_137400483.1	amidohydrolase/deacetylase family metallohydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-83)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-80)

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 10868.70 Da
• Isoelectric Point: 10.0482

>T163684 WP_000220581.1 NZ_CP056559:c4179138-4178854 [Citrobacter freundii]
MTYELEFDPRALKEWHKLGDTVKAQLKKKLADVLLNPRIDSARLNGLPDCYKIKLKSSGYRLVYQVRDEVVIVFVVAVGK
REHSAVYHDANKRL

Nucleotide

• Length: 285 bp

>T163684 NZ_CP072761:c1575877-1575476 [Mycobacterium tuberculosis]
ATGATCCTTGTCGACTCCGATGTGCTGATCGCGCATTTGCGGGGTGTCGTTGCTGCTCGCGATTGGCTTGTCAGCGCCCG
CAAGGACGGACCGCTGGCGATCAGCGTGGTGTCCACCGCCGAACTCATCGGCGGAATGCGGACCGCCGAACGGCGCGAGG
TGTGGCGCCTGCTTGCATCGTTTCGGGTACAGCCAGCAACCGAGGTAATCGCACGCCGCGCCGGCGACATGATGCGCCGA
TATCGTCGCAGCCACAACCGGATTGGACTTGGCGACTATCTGATAGCTGCTACCGCCGACGTCCAGGACCTGCAATTGGC
AACCCTCAACGTGTGGCATTTCCCCATGTTCGAGCAGCTGAAACCACCATTTGCGGTGCCGGGGCACCGACCGCGGGCAT
GA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8947.32 Da
• Isoelectric Point: 5.0490

>AT163684 WP_115259576.1 NZ_CP056559:c4179370-4179128 [Citrobacter freundii]
MATLNVRLDDKLKNEAYAVLDKLNITPTEAVRLLFQYVAETGRMPVKTVTLSDSEDALIQTVRERLSSPQKGIKVSLDDL

Nucleotide

• Length: 243 bp

>AT163684 NZ_CP072761:c1576131-1575874 [Mycobacterium tuberculosis]
ATGAAGCGGACCAACATCTACCTCGACGAGGAGCAGACGGCAAGCCTCGACAAGTTGGCCGCGCAAGAAGGTGTTTCGCG
CGCCGAGCTGATCCGCCTCCTGCTGAACCGAGCCCTCACCACCGCTGGGGACGACCTTGCATCGGACCTGCAGGCTATAA
ACGATTCGTTCGGCACGCTTCGCCACCTGGATCCGCCGGTGCGTCGCTCCGGTGGTCGTGAACAGCACCTTGCCCAGGTG
TGGCGCGCCACCTCATGA

Structures

Toxin

• AlphaFold DB: A0A5I6CHB9