Detailed information of TA system TA163683

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 4067527..4068103
• Replicon: chromosome
• Accession: NZ_CP056559
• Organism: Citrobacter freundii strain RHBSTW-00408

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: -
• Locus tag: HV186_RS19340
• Protein ID: WP_181490627.1
• Coordinates: 4067816..4068103 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: -
• Locus tag: HV186_RS19335
• Protein ID: WP_117342368.1
• Coordinates: 4067527..4067829 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV186_RS19320	4063995..4066145	+	2151	WP_135912629.1	pyruvate/proton symporter BtsT	-
HV186_RS19325	4066253..4066447	+	195	WP_181490625.1	YbdD/YjiX family protein	-
HV186_RS19330	4066460..4067416	+	957	WP_181490626.1	GTPase	-
HV186_RS19335	4067527..4067829	-	303	WP_117342368.1	BrnA antitoxin family protein	Antitoxin
HV186_RS19340	4067816..4068103	-	288	WP_181490627.1	BrnT family toxin	Toxin
HV186_RS19345	4068276..4068440	-	165	WP_117342370.1	DUF1127 domain-containing protein	-
HV186_RS19350	4068617..4070029	+	1413	WP_181218811.1	PLP-dependent aminotransferase family protein	-
HV186_RS19355	4070056..4070652	-	597	Protein_3788	Rpn family recombination-promoting nuclease/putative transposase	-
HV186_RS19360	4071099..4072340	+	1242	WP_135912631.1	multidrug efflux MFS transporter MdtM	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (42-93)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11392.77 Da
• Isoelectric Point: 7.5239

>T163683 WP_181490627.1 NZ_CP056559:c4068103-4067816 [Citrobacter freundii]
MPMEFEWDANKAQSNHRKHGVRFEDAVLVFDDPRHLSRQDRYENGEYRWQTIGLVHGIVVILVTHSVRFESGFEVIRIIS
ARKADRKERNRYEHD

Nucleotide

• Length: 288 bp

>T163683 NZ_CP072761:1539754-1540575 [Mycobacterium tuberculosis]
GTGGTGGTAGCCCTCGTAGGCTCAGCAATCGTGGACTTGCATTCACGACCACCGTGGTCGAACAACGCGGTGCGTCGTCT
TGGCGTGGCACTGCGCGACGGAGTTGACCCGCCGGTCGACTGCCCGTCGTACGCCGAGGTGATGCTGTGGCATGCGGACT
TGGCCGCCGAAGTCCAGGACCGGATCGAGGGCCGGAGTTGGTCTGCGTCGGAGTTATTGGTTACCTCACGTGCGAAGAGC
CAAGACACCCTGCTAGCAAAGCTGCGGCGTCGGCCTTACCTGCAACTGAACACCATCCAAGACATCGCAGGTGTCCGCAT
CGATGCCGACCTCCTGCTGGGCGAGCAGACGAGACTTGCTCGCGAGATCGCCGACCACTTCGGTGCTGACCAGCCCGCTA
TTCATGATCTGCGTGACCACCCGCACGCCGGCTACCGGGCCGTTCATGTCTGGCTTCGGTTACCTGCCGGTCGTGTCGAG
ATACAGATTCGCACCATTTTGCAGAGCCTGTGGGCCAACTTCTACGAGCTTCTCGCTGACGCGTACGGTCGGGGCATCCG
CTATGACGAGCGGCCGGAGCAGCTAGCGGCCGGCGTTGTCCCGGCACAGCTTCAAGAGCTGGTAGGGGTTATGCAAGACG
CTTCAGCGGATCTGGCGATGCATGAAGCCGAGTGGCAACACTGTGCAGAGATCGAATACCCCGGCCAGCGGGCGATGGCG
CTTGGCGAGGCGAGCAAGAACAAGGCGACGGTGCTCGCAACGACCAAGTTTAGGCTGGAAAGGGCCATCAATGAGGCCGA
GTCGGCAGGGGGAGGTGGGTGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11444.95 Da
• Isoelectric Point: 8.5366

>AT163683 WP_117342368.1 NZ_CP056559:c4067829-4067527 [Citrobacter freundii]
MSMIKHKRGSASALSAQHEAELKALANKPDDDIDYSDIPVTEDEQWSEANRGTFFRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLREQNKK

Nucleotide

• Length: 303 bp

>AT163683 NZ_CP072761:1540568-1540804 [Mycobacterium tuberculosis]
GTGGGTGAGGTGGCTGGCTATGTCGTCGAATACAACCGGCGCACCCACGTGCGTCGCATCACCGAGTTCGCCACCCCGCA
AGAAGCGATGGAGCACCGGTTGAAGCTGGAAGCCGAGCGCACCGACAGCAATATCGAGATCGTTGCGCTCGTCAGTAAGT
CGTTGGGAACCCTGAAGCAAACGCATTCGCGGTACTTCACTGGTGAAGAGCTGAACGTCGGAAACGGCGCGCGGTAG