Detailed information of TA system TA163667

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 84229..84965
• Replicon: plasmid pRHBSTW-00422_2
• Accession: NZ_CP056553
• Organism: Enterobacter sp. RHBSTW-00422

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: A0A2J5Q928
• Locus tag: HV189_RS25215
• Protein ID: WP_004098919.1
• Coordinates: 84483..84965 (+)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: A0A2J5Q9C0
• Locus tag: HV189_RS25210
• Protein ID: WP_004098921.1
• Coordinates: 84229..84495 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV189_RS25165	79412..80413	+	1002	WP_009654304.1	Abi family protein	-
HV189_RS25170	80552..81130	-	579	WP_009654302.1	hypothetical protein	-
HV189_RS25175	81420..81683	+	264	WP_009310051.1	hypothetical protein	-
HV189_RS25180	81680..82246	+	567	WP_063858698.1	hypothetical protein	-
HV189_RS25185	82277..82771	+	495	WP_013023776.1	hypothetical protein	-
HV189_RS25190	82815..83183	+	369	WP_042946273.1	hypothetical protein	-
HV189_RS25195	83214..83417	+	204	WP_004098931.1	hemolysin expression modulator Hha	-
HV189_RS25200	83466..83723	+	258	WP_004098928.1	hypothetical protein	-
HV189_RS25205	83799..84053	+	255	WP_004118702.1	hypothetical protein	-
HV189_RS25210	84229..84495	+	267	WP_004098921.1	DUF1778 domain-containing protein	Antitoxin
HV189_RS25215	84483..84965	+	483	WP_004098919.1	GNAT family N-acetyltransferase	Toxin
HV189_RS25220	85166..86569	+	1404	WP_001567368.1	ISNCY-like element ISKpn21 family transposase	-
HV189_RS25225	86598..87230	-	633	WP_001567369.1	hypothetical protein	-
HV189_RS25230	87407..88939	-	1533	WP_064386399.1	IS3 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	mrkJ / mrkF / mrkD / mrkC / mrkB / mrkA	1..163659	163659

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (66-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17335.06 Da
• Isoelectric Point: 10.0704

>T163667 WP_004098919.1 NZ_CP056553:84483-84965 [Enterobacter sp. RHBSTW-00422]
VGRITAPEPLSSAHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKKGTKKVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Nucleotide

• Length: 483 bp

>T163667 NZ_CP072761:c697570-697157 [Mycobacterium tuberculosis]
GTGAAACCGCCGCTGGCAGTGGACACCAGCGTCGCGATTCCGTTGCTGGTACGGACCCATACCGCGCATGCGGCGGTTGT
CGCCTGGTGGGCACATCGGGAGGCCGCGCTGTGCGGGCACGCCCTGGCCGAAACCTACTCCGTGCTGACCCGCCTGCCGC
GTGACCTACGACTGGCACCGATGGACGCCGCGCGGCTTCTTACCGAACGCTTTGCGGCACCGCTGTTGTTGAGTTCCCGC
ACCACTGAACATCTCCCACGCGTGCTGGCCCAATTCGAGATCACTGGTGGCGCGGTCTATGACGCTCTCGTGGCCCTTGC
CGCCGCCGAGCACCGGGCTGAACTCGCCACACGCGATGCTCGAGCCAAGGACACTTACGAAAAGATCGGCGTCCACGTCG
TTGTCGCCGCGTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10105.49 Da
• Isoelectric Point: 4.5141

>AT163667 WP_004098921.1 NZ_CP056553:84229-84495 [Enterobacter sp. RHBSTW-00422]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEIACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDDSAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT163667 NZ_CP072761:c697803-697567 [Mycobacterium tuberculosis]
ATGAAGGCTGTTGTGGATGCGGCTGGCCGCATCGTCGTACCCAAACCGCTGCGCGAAGCCTTGGGGCTGCAACCGGGGTC
CACCGTGGAAATATCCCGCTATGGCGCTGGTCTCCACCTGATTCCCACCGGACGAACAGCGCGTCTCGAAGAGGAGAATG
GCGTACTGGTGGCCACCGGTGAGACCACGATCGACGATGAGGTCGTGTTCGGCCTTATCGATAGCGGCCGGAAGTGA

Structures

Toxin

• AlphaFold DB: A0A2J5Q928

Antitoxin

• AlphaFold DB: A0A2J5Q9C0