Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/ElaA-DUF1778
Location 46894..47697 Replicon plasmid pRHBSTW-00424_2
Accession NZ_CP056547
Organism Citrobacter sp. RHBSTW-00424

Toxin (Protein)


Gene name KacT Uniprot ID A0A7W3E3C9
Locus tag HV191_RS26395 Protein ID WP_022652313.1
Coordinates 47167..47697 (+) Length 177 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID W8E6Q5
Locus tag HV191_RS26390 Protein ID WP_022652312.1
Coordinates 46894..47163 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HV191_RS26375 43199..43506 - 308 Protein_45 IS1 family transposase -
HV191_RS26380 43983..44723 + 741 WP_022652310.1 site-specific integrase -
HV191_RS26385 45049..46038 - 990 WP_022652311.1 RepB family plasmid replication initiator protein -
HV191_RS26390 46894..47163 + 270 WP_022652312.1 DUF1778 domain-containing protein Antitoxin
HV191_RS26395 47167..47697 + 531 WP_022652313.1 GNAT family N-acetyltransferase Toxin
HV191_RS26400 47829..48722 + 894 WP_176311857.1 IS5 family transposase -
HV191_RS26405 48863..49339 - 477 Protein_51 IS3 family transposase -
HV191_RS26410 49420..50928 - 1509 WP_001189111.1 group II intron reverse transcriptase/maturase -
HV191_RS26415 51601..52030 - 430 Protein_53 IS3 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..119062 119062
- inside IScluster/Tn - - 41885..55091 13206


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(92-156)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 177 a.a.        Molecular weight: 20349.26 Da        Isoelectric Point: 6.7496

>T163655 WP_022652313.1 NZ_CP056547:47167-47697 [Citrobacter sp. RHBSTW-00424]
MEGLRIEIFSEEVEYELSNFDCGEEYLNTFLTDHLKRQHNSKILRGYVLVTRENKPRVMGYYTLSGSCFEKILLPSKTQQ
KRVPYKNVPSVTLGRLAIDKSIHHQGYGETLVTHAMKVVYQASQAVGIHGMFVEALNDNAKKFYLRLGFIQLKEENCNSL
FYPTKSIEELFEVNDE

Download         Length: 531 bp

>T163655 NZ_CP072742:3782405-3782758 [Serratia fonticola]
GTGTGGGAGATTAAAACAACGGATACGTTTGACGAGTGGTTCAGCCGGTTGGAGGATACTGATAGAGCTTCTGTGCTGGC
GTCACTCATTGTACTACGTGAAAAAGGTCCTCAGTTGCCCAGACCCTATGCCGACACGGTAAAGGGATCGCGCTACAGCA
ACATGAAGGAACTACGTGTGCAAAGCCGAGGCATACCGATAAGGGCCTTCTTTGCCTTTGATCCACGCCGTCGCGGTATT
TTGCTTTGTGCTGGTAACAAGGCTGGTAATGAGAAGCGTTTTTATGAGGTGATGATCCCGATTGCCGATCGGGAATTTAC
CCAATTTCTGAATACAGCAGAGAACAAGGAGTAG

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10269.70 Da        Isoelectric Point: 5.7252

>AT163655 WP_022652312.1 NZ_CP056547:46894-47163 [Citrobacter sp. RHBSTW-00424]
MSALKKQRIDLRLNEDDKHMIEEAAAMTNQSISQFMVSTASERAAEVIDQHRRLLLNEESWNLVMDAIINPPAPNDRLKR
AANRLRELE

Download         Length: 270 bp

>AT163655 NZ_CP072742:3782763-3783065 [Serratia fonticola]
ATGGGCAGAACACTGGAACAAATCCTGGCGGAAGAAAAACCCGAGGTAGTGGCCAGCGCTGAGCGCATTGCGGCGGATAC
GCTGCTCAATATTCACCTGGCCGAGCTACGTGAGCGGGTGCAGAAAACCCAGGTTGAAATGGCGCAGGCACTTGGCATCA
AACAACCCACCGTGGCGGGCATGGAAAAACCAGGCCGCGATCTGAAGCTCTCCTCGCTCAAACGCTATATCGAGGCCGCG
GGGGGCAAGCTGCGTCTGGATATAGAGCTGCCAGATGGTTCGCACTATGGGTTTGTTATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7W3E3C9

Antitoxin

Source ID Structure
AlphaFold DB A0A2Z3XDS5

References