Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5187310..5187926 Replicon chromosome
Accession NZ_CP056546
Organism Citrobacter sp. RHBSTW-00424

Toxin (Protein)


Gene name vapC Uniprot ID A0A2I8SCX0
Locus tag HV191_RS25290 Protein ID WP_049269424.1
Coordinates 5187310..5187684 (-) Length 125 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID R8WIS4
Locus tag HV191_RS25295 Protein ID WP_016155181.1
Coordinates 5187684..5187926 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HV191_RS25275 5184813..5185715 + 903 WP_003028691.1 formate dehydrogenase O subunit beta -
HV191_RS25280 5185712..5186347 + 636 WP_003028686.1 formate dehydrogenase cytochrome b556 subunit -
HV191_RS25285 5186344..5187273 + 930 WP_003028685.1 formate dehydrogenase accessory protein FdhE -
HV191_RS25290 5187310..5187684 - 375 WP_049269424.1 type II toxin-antitoxin system VapC family toxin Toxin
HV191_RS25295 5187684..5187926 - 243 WP_016155181.1 ribbon-helix-helix domain-containing protein Antitoxin
HV191_RS25300 5188132..5189040 + 909 WP_049269423.1 alpha/beta hydrolase -
HV191_RS25305 5189105..5189347 + 243 WP_016155179.1 type II toxin-antitoxin system ParD family antitoxin -
HV191_RS25310 5189340..5189627 + 288 WP_049269420.1 type II toxin-antitoxin system RelE/ParE family toxin -
HV191_RS25315 5189633..5190574 - 942 WP_003825286.1 fatty acid biosynthesis protein FabY -
HV191_RS25320 5190619..5191056 - 438 WP_003028671.1 D-tyrosyl-tRNA(Tyr) deacylase -
HV191_RS25325 5191053..5191925 - 873 WP_019077938.1 virulence factor BrkB family protein -
HV191_RS25330 5191919..5192448 - 530 Protein_4964 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-113)

Antitoxin

Ribbon-helix-helix protein, CopG

(13-47)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13650.78 Da        Isoelectric Point: 8.5373

>T163654 WP_049269424.1 NZ_CP056546:c5187684-5187310 [Citrobacter sp. RHBSTW-00424]
MATGSALFDTNILIDLFSGRSEAKLAIETWPPQNAISLITWMELMVGAKKYHQESRTRVALGAFNVIGVSQDIAERSVSI
RQEYGMKLPDAIILATAQIHRLTLVTRNTRDFAGISGVVTPYTL

Download         Length: 375 bp

>T163654 NZ_CP072742:3262128-3262517 [Serratia fonticola]
ATGTATATGTTCGACACTAACACGGTGAGCCAGCTTTTTCGTCAACATCCCCGGCTGCTTGCGGCTATGGGCCGGGTACC
GCCATCGTCGGTGTGTATTTCCAGCATAACCCAGGCGGAACTGCTTTATGGTGTCGCCAAGCGGCAGAACAAGGCGCTAA
AACAGACGGTGATGATGTTCCTGGACGCCGTCACCGTTTACTCCTGGGACTCTGCGGCGGCGCATTGCTATGGTGTCATG
CGCGCAAGTATGGAAAAACAAGGCAGACCGATGGGGGCGTTGGATCAGCTTATTGCCGCACATGCGGTAAGCCGTGGCGC
AACCATAGTGACCAGCGATCGGGCTTTCACTATGGTCGCGGAGCTTGAGGTGGAAGACTGGACGCTGTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9437.72 Da        Isoelectric Point: 4.3331

>AT163654 WP_016155181.1 NZ_CP056546:c5187926-5187684 [Citrobacter sp. RHBSTW-00424]
MMAGMDMGRILLDLSDEVIKRLDDLKVQRNLPRAELLREAVEQYLERQDQAETTISRALGLWQGCEEDGVDYQRKLREEW

Download         Length: 243 bp

>AT163654 NZ_CP072742:3261861-3262124 [Serratia fonticola]
ATGGAAAGAGTCGCCAAACTTTTTAAAAACGGTAGAAATCAGGCGGTGAGATTGCCAGTGGAATTCGAATTCGATGTCGA
TCAGGTTTATATTCGCCGGGATGAACAGGGTAACGTTATCTTGTCGAAGCATCCAATGAAATTGGACTCCTGGCAACCGC
TACTGACCATGCTGCAAAACGTGGCGGTTCCTGCTGACTTCCTGACTGCTGACGATCGCCAGCAAGGGGTAGCAGAACGG
GATCCGTTCAAGGAGGAACAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2I8SCX0

Antitoxin

Source ID Structure
AlphaFold DB R8WIS4

References