163651

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yafW-ykfI/CbtA-CbeA
Location 4003923..4004602 Replicon chromosome
Accession NZ_CP056546
Organism Citrobacter sp. RHBSTW-00424

Toxin (Protein)


Gene name ykfI Uniprot ID A0A2I8S2T2
Locus tag HV191_RS19540 Protein ID WP_008324514.1
Coordinates 4004261..4004602 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name yafW Uniprot ID A0A2I8S2U6
Locus tag HV191_RS19535 Protein ID WP_008324516.1
Coordinates 4003923..4004240 (+) Length 106 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HV191_RS19490 3999364..4000185 + 822 WP_032654809.1 DUF945 domain-containing protein -
HV191_RS19495 4000403..4001104 + 702 WP_008324530.1 WYL domain-containing protein -
HV191_RS19500 4001141..4001377 + 237 WP_001115854.1 hypothetical protein -
HV191_RS19505 4001377..4001820 + 444 WP_000824223.1 hypothetical protein -
HV191_RS19510 4001843..4002310 + 468 WP_001547765.1 hypothetical protein -
HV191_RS19515 4002387..4002626 + 240 WP_000194654.1 DUF905 domain-containing protein -
HV191_RS19520 4002724..4003182 + 459 WP_000211838.1 antirestriction protein -
HV191_RS19525 4003198..4003674 + 477 WP_000811693.1 RadC family protein -
HV191_RS19530 4003683..4003910 + 228 WP_008324517.1 DUF987 domain-containing protein -
HV191_RS19535 4003923..4004240 + 318 WP_008324516.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
HV191_RS19540 4004261..4004602 + 342 WP_008324514.1 type IV toxin-antitoxin system toxin YkfI Toxin
HV191_RS19545 4004808..4005928 + 1121 WP_103283453.1 IS3 family transposase -
HV191_RS19555 4006450..4006914 - 465 WP_103283511.1 isoprenylcysteine carboxylmethyltransferase family protein -
HV191_RS19560 4007108..4008556 - 1449 WP_103283510.1 EAL domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 3969214..4027926 58712


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(6-104)


Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 12919.98 Da        Isoelectric Point: 9.6543

>T163651 WP_008324514.1 NZ_CP056546:4004261-4004602 [Citrobacter sp. RHBSTW-00424]
MKTLPATTQRAAKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
NWQEQSPYLRAVDILRARQATGLLRQSRNNVVR

Download         Length: 342 bp

>T163651 NZ_CP072742:c2525040-2524759 [Serratia fonticola]
ATGACTTATAAGCTCAAATTTGAAAAGCGCGCACTGAAGGAATGGGAAAAGCTGGGTTATCCCGTTCGGGAACAACTGAA
AAAGAAGCTGGCCGAGCGTTTAGAAAACCCGCATGTGCCGGCATCCCGCCTAAGTGGCCGCCCGAACCGTTACAAAATCA
AACTGCGCTCATCAGGCTATCGGTTGGTTTATGAAGTACGTGATAATGAAATTGTTCTATTGGTGATCGCCGTTGGAAAA
CGGGCTGGGGAGGAAGTCTATTTTGCTGCCGATAAGCGATAG

Antitoxin


Download         Length: 106 a.a.        Molecular weight: 11787.33 Da        Isoelectric Point: 7.3476

>AT163651 WP_008324516.1 NZ_CP056546:4003923-4004240 [Citrobacter sp. RHBSTW-00424]
MSNPTRGLQRAITPRLGARLVQEGNRLHYLADRASITGRFSDTECRKLDETCPHFIRHMESMLTTGELSPQHAHCVTLYH
NGFTCEADTLGSCGYVYIAIYPTQS

Download         Length: 318 bp

>AT163651 NZ_CP072742:c2525281-2525030 [Serratia fonticola]
ATGGCCTTTCAAATTTTAACCACTATCGCTGCCAGCATCACCGACCTGAAAAAGAACCCGATGGGAACCGTTGCAGACGG
AGAAGGTGAAGCGGTTGCCATCCTGAATCGTAACGAACCGGCTTTCTACTGCGTGCCCCCTGAGCTGTATGCATACTATC
GGGAGCTTGCCGAAGATGCAGAATTAAATCGTATTGCAGATGAACGCATGGAAGATGCGGAGTTTGTGAGCGTAGATCTT
AATGACTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2I8S2T2

Antitoxin

Source ID Structure
AlphaFold DB A0A2I8S2U6

References