Detailed information of TA system TA163648

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2709724..2710360
• Replicon: chromosome
• Accession: NZ_CP056546
• Organism: Citrobacter sp. RHBSTW-00424

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A2I8S6E9
• Locus tag: HV191_RS13380
• Protein ID: WP_049259794.1
• Coordinates: 2710172..2710360 (-)
• Length: 63 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: HV191_RS13375
• Protein ID: WP_146053235.1
• Coordinates: 2709724..2710140 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV191_RS13355	2704769..2705713	-	945	WP_016153152.1	ABC transporter permease	-
HV191_RS13360	2705714..2706730	-	1017	WP_016153153.1	ABC transporter ATP-binding protein	-
HV191_RS13365	2706747..2707892	-	1146	WP_016153154.1	ABC transporter substrate-binding protein	-
HV191_RS13370	2708222..2709631	-	1410	WP_103283798.1	PLP-dependent aminotransferase family protein	-
HV191_RS13375	2709724..2710140	-	417	WP_146053235.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
HV191_RS13380	2710172..2710360	-	189	WP_049259794.1	type II toxin-antitoxin system HicA family toxin	Toxin
HV191_RS13385	2710624..2712027	+	1404	WP_016153156.1	cytochrome ubiquinol oxidase subunit I	-
HV191_RS13390	2712027..2713037	+	1011	WP_103283796.1	cytochrome d ubiquinol oxidase subunit II	-
HV191_RS13395	2713037..2713162	+	126	WP_003020230.1	DUF2474 domain-containing protein	-
HV191_RS13400	2713352..2713582	+	231	WP_016156585.1	DUF2554 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-64)

Sequences

Toxin

Protein

• Length: 63 a.a.
• Molecular weight: 7102.16 Da
• Isoelectric Point: 11.5775

>T163648 WP_049259794.1 NZ_CP056546:c2710360-2710172 [Citrobacter sp. RHBSTW-00424]
VKQSEFRRWLESQGVAVTNGSNHLKLRYQGRRSVMPRHPGDEIKEALRKSILKQLGLQSSSI

Nucleotide

• Length: 189 bp

>T163648 NZ_CP072742:c1556428-1556078 [Serratia fonticola]
TTGTGGACTGTTATTTTTACCTCGACATTTGATTCTTGGTTGCAAGAGCAGGAAGAAGGGATGCAGGAGAAGGTACTGGC
TGACCTGATGAACCTGGAGACGTATGGGCCTCAACTGCCACGTCCTTACGCTGACACGGTAAAAGGTTCAAGATACAAGA
ATATGAAAGAGCTGCGTGTTCAGTATTCGGGCCGCCCAATCCGTGCCTTTTTTGTTTTCGACCCCAAACGCCAAGCCATT
GTGTTGTGCGCTGGTGATAAAAGTAATGATAAGCGTTTTTACGAGACAATGATCCGAATAGCGGATGATGAATTTACGGC
GTATTTAGCCACACAAGAGGTATCCAAATGA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15080.33 Da
• Isoelectric Point: 4.7119

>AT163648 WP_146053235.1 NZ_CP056546:c2710140-2709724 [Citrobacter sp. RHBSTW-00424]
MRYPVNLIPSVEGGFVVSFPDIPEALTQGETRHGALQAAQSALVTAFEFYFDDNEPIPLPSAVGTEGDYVEIPLSIASKV
LLLNAFLESKITQQELANRIGRPKQEITRLFDLKHATKIDAVQIAARALGKELSVTML

Nucleotide

• Length: 417 bp

>AT163648 NZ_CP072742:c1556081-1555782 [Serratia fonticola]
ATGAAAACGTTACGTGATGCAGTCGCTGCCCGTTCTCTTGAGAGCCAAGCCCGTATCAAGGAAATGGCCGACGAAATGAT
GCTAGAAACAGGTTTGCAACTGATGCGTGATGAGCTGCAACTGTCACAGAAAAGCCTGGCCGAAGCTATGGGCGTAAGCC
AATCCGCGGTTGCACAAATAGAGCAGCGTGGTAATGACGTAAAGTTAGCGACGCTAAAACGCTATATTGAAGCTATGGGT
GGGAAGTTGAGTCTGACTGTAGAGATGCCGGAAGGCGGTGGGCGAGTCTTCCATATCTGA

Structures

Toxin

• AlphaFold DB: A0A2I8S6E9