Detailed information of TA system TA163639

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): cptAB/Cpta(toxin)
• Location: 907205..907859
• Replicon: chromosome
• Accession: NZ_CP056546
• Organism: Citrobacter sp. RHBSTW-00424

Toxin (Protein)

• Gene name: cptA
• Uniprot ID: R8WN60
• Locus tag: HV191_RS04460
• Protein ID: WP_016154348.1
• Coordinates: 907452..907859 (+)
• Length: 136 a.a.

Antitoxin (Protein)

• Gene name: cptB
• Uniprot ID: R8WMJ6
• Locus tag: HV191_RS04455
• Protein ID: WP_016154349.1
• Coordinates: 907205..907471 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV191_RS04430	902418..903851	-	1434	WP_016154353.1	6-phospho-beta-glucosidase BglA	-
HV191_RS04435	903972..904700	-	729	WP_103284472.1	MurR/RpiR family transcriptional regulator	-
HV191_RS04440	904753..905064	+	312	WP_016154352.1	N(4)-acetylcytidine aminohydrolase	-
HV191_RS04445	905228..905887	+	660	WP_016154351.1	hemolysin III family protein	-
HV191_RS04450	905967..906947	-	981	WP_016154350.1	tRNA-modifying protein YgfZ	-
HV191_RS04455	907205..907471	+	267	WP_016154349.1	FAD assembly factor SdhE	Antitoxin
HV191_RS04460	907452..907859	+	408	WP_016154348.1	protein YgfX	Toxin
HV191_RS04465	907960..908481	-	522	WP_016154347.1	flavodoxin FldB	-
HV191_RS04470	908595..909491	+	897	WP_016154346.1	site-specific tyrosine recombinase XerD	-
HV191_RS04475	909515..910228	+	714	WP_075847456.1	bifunctional protein-disulfide isomerase/oxidoreductase DsbC	-
HV191_RS04480	910234..911967	+	1734	WP_103284192.1	single-stranded-DNA-specific exonuclease RecJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Inner membrane protein YgfX (3-131)

Antitoxin

• Flavinator of succinate dehydrogenase (7-79)

Sequences

Toxin

Protein

• Length: 136 a.a.
• Molecular weight: 15850.66 Da
• Isoelectric Point: 11.2845

>T163639 WP_016154348.1 NZ_CP056546:907452-907859 [Citrobacter sp. RHBSTW-00424]
VVQWQSDLRVSWRAQWLSLLIHGLVAVFILLMPWPLSYTPLWLVLLSLVVFDSVRSQRRINACQGEIRLLMDGRLRWQGQ
EWTIASAPWMVKTGMMLRLRSDAGKRQHLWLAADSMDDAEWRDLRRLILQQAKQG

Nucleotide

• Length: 408 bp

>T163639 NZ_CP072731:194240-194506 [Enterococcus faecium]
ATGAGTAACTATACAGTCGCAATCAAAAACTCTGCGAAAGTGGATTTAAGAAAAATCAAGCAAACGAATTTAAAGAAACA
ATTTGAAGAAGTGATTCAGACATTGAAGGAAGATCCATATATGCCCACGCAGTCTTTTGAAAAGCTGAGACCTACACATG
AAGGCAGATACTCACGCAGGCTGAATAGACAGCATCGAGTGGTTTACAAAGTTGATGAGGAAAATAAAGTTGTAGAAATT
TATTCTGCCTGGACACATTATGAGTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10557.10 Da
• Isoelectric Point: 5.6375

>AT163639 WP_016154349.1 NZ_CP056546:907205-907471 [Citrobacter sp. RHBSTW-00424]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDEEKRIFIRLLECDDPDLFNWLMNHGKPADAQLEQMVRLIQTRN
RERGPVAI

Nucleotide

• Length: 267 bp

>AT163639 NZ_CP072731:193977-194240 [Enterococcus faecium]
ATGGCTAATACAACGATGAATCCTAGCACTGCTCGTAAAAATTTTTATCAGTTATTGAAAGAGGTAAATGAAAATCATAC
TGAAATCGAGATTATTAGTGATCGTAGTGGAAACAATGCTGTATTGATTGGGCTGGAAGACTGGAGAGCGATTCAAGAAA
CCCTTTTTCTTGAGCAAACAGGTACTTTAGATAAAGTACGAGACCGCGAAAAAGACGACAGTAGCTTTACGAATATCGAT
GATATTGATTGGGAAGCTCTTTGA

Structures

Toxin

• AlphaFold DB: R8WN60

Antitoxin

• AlphaFold DB: R8WMJ6