Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 20777..21302 Replicon plasmid pRHBSTW-00433_2
Accession NZ_CP056531
Organism Klebsiella pneumoniae strain RHBSTW-00433

Toxin (Protein)


Gene name ccdB Uniprot ID A0A7H9GHC5
Locus tag HV193_RS25600 Protein ID WP_009309918.1
Coordinates 20777..21082 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID A0A1D8K3R4
Locus tag HV193_RS25605 Protein ID WP_006788213.1
Coordinates 21084..21302 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HV193_RS25565 15939..16907 + 969 WP_000654811.1 IS5 family transposase -
HV193_RS25570 17439..17552 + 114 WP_014343462.1 hypothetical protein -
HV193_RS25575 17681..17938 + 258 WP_009310077.1 hypothetical protein -
HV193_RS25580 17996..18775 - 780 WP_023287113.1 site-specific integrase -
HV193_RS25585 18973..19989 - 1017 WP_009309921.1 hypothetical protein -
HV193_RS25590 20023..20358 - 336 WP_009309920.1 hypothetical protein -
HV193_RS25595 20408..20719 - 312 WP_009309919.1 hypothetical protein -
HV193_RS25600 20777..21082 - 306 WP_009309918.1 type II toxin-antitoxin system toxin CcdB Toxin
HV193_RS25605 21084..21302 - 219 WP_006788213.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
HV193_RS25610 21899..22129 + 231 WP_001261276.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
HV193_RS25615 22126..22542 + 417 WP_020804312.1 type II toxin-antitoxin system VapC family toxin -
HV193_RS25620 22583..23461 - 879 WP_009309916.1 restriction endonuclease -
HV193_RS25625 24076..25146 - 1071 WP_181469602.1 IS481-like element ISKpn28 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - mrkA / mrkB / mrkC / mrkD / mrkF / mrkJ 1..224837 224837


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11571.24 Da        Isoelectric Point: 6.4661

>T163635 WP_009309918.1 NZ_CP056531:c21082-20777 [Klebsiella pneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVVPLASARLLSDKVSRELYPVVHIGDDSYRLMTTDMASVTASVTGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T163635 NZ_CP072713:7923-8180 [Enterococcus faecium]
ATGATTAAGGCTTGGTCTGATGATGCTTGGGATGATTATCTTTATTGGCATGAGCAAGGAAACAAAAGCAATATAAAAAA
GATTAACAAGTTAATAAAAGATATCGATCGTTCCCCCTTTGCTGGATTAGGAAAACCTGAGCCATTAAAGCATGATTTAT
CTGGAAAATGGTCCAGAAGAATTACAGATGAACATAGACTGATATATAGAGTTGAAAATGAAACGATATTTATTTATTCT
GCAAAAGATCACTATTAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8362.31 Da        Isoelectric Point: 4.7405

>AT163635 WP_006788213.1 NZ_CP056531:c21302-21084 [Klebsiella pneumoniae]
MKQRITVTIDSDSYQLLKSADVNISGLVNRAMQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT163635 NZ_CP072713:7661-7930 [Enterococcus faecium]
ATGGAAGCAGTAGCTTATTCAAATTTCCGCCAAAATTTACGTAGTTATATGAAACAAGTTAATGAGGATGCTGAAACACT
TATTGTAACAAGTAAAGATGTAGAAGATACAGTTGTTGTATTATCAAAAAGAGATTATGATTCTATGCAAGAAACGTTGA
GAACACTTTCTAATAATTACGTCATGGAAAAAATTCGTCGAGGAGATGAACAATTCTCCAAAGGTGCATTTAAAACACAT
GACTTAATCGAGGTTGAATCTGATGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7H9GHC5

Antitoxin

Source ID Structure
AlphaFold DB A0A1D8K3R4

References