Detailed information of TA system TA163631

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): kacAT/DUF1778(antitoxin)
• Location: 4586836..4587646
• Replicon: chromosome
• Accession: NZ_CP056530
• Organism: Klebsiella pneumoniae strain RHBSTW-00433

Toxin (Protein)

• Gene name: KacT
• Uniprot ID: -
• Locus tag: HV193_RS22375
• Protein ID: WP_060416080.1
• Coordinates: 4586836..4587369 (-)
• Length: 178 a.a.

Antitoxin (Protein)

• Gene name: KacA
• Uniprot ID: J2E9Q7
• Locus tag: HV193_RS22380
• Protein ID: WP_002887278.1
• Coordinates: 4587380..4587646 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV193_RS22370	4585667..4586788	+	1122	WP_060416081.1	cupin domain-containing protein	-
HV193_RS22375	4586836..4587369	-	534	WP_060416080.1	GNAT family N-acetyltransferase	Toxin
HV193_RS22380	4587380..4587646	-	267	WP_002887278.1	DUF1778 domain-containing protein	Antitoxin
HV193_RS22385	4587749..4589182	-	1434	WP_060416079.1	Cu(+)/Ag(+) sensor histidine kinase	-
HV193_RS22390	4589172..4589855	-	684	WP_004214143.1	copper response regulator transcription factor CusR	-
HV193_RS22395	4590027..4591412	+	1386	WP_060416078.1	efflux transporter outer membrane subunit	-
HV193_RS22400	4591430..4591774	+	345	WP_025987660.1	cation efflux system protein CusF	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Vibrio phage ICP1, Orf50 (2-86)

Sequences

Toxin

Protein

• Length: 178 a.a.
• Molecular weight: 19699.56 Da
• Isoelectric Point: 5.2466

>T163631 WP_060416080.1 NZ_CP056530:c4587369-4586836 [Klebsiella pneumoniae]
MEQQLTIEMIAGAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLCLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Nucleotide

• Length: 534 bp

>T163631 NZ_CP072708:93857-94009 [Enterococcus faecium]
ATTGAAAAAAATGGCTGGGTTGAGCGAAGGCAAGAGGGATTTCATCATCATCTTTATAAGGATGGTGTTAGAATCACTGT
TCCTGTTCATGCCAATCAAGATCTTGGCCGAGGACTTGAGCGAAAAATTTTAAAGGATGCGGGATTGAAATAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9996.46 Da
• Isoelectric Point: 10.1757

>AT163631 WP_002887278.1 NZ_CP056530:c4587646-4587380 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Nucleotide

• Length: 267 bp

>AT163631 NZ_CP072708:94043-94474 [Enterococcus faecium]
ATGTTAAGTGAGCCTTTAGCAAAAACCTATCCTGCTATTTTTAGCCCAGAAGAAGGTGGTGGGTACTTTATTGAATTTCC
TGATGTTCAAGGTGCTTACACAGGAATTAACGAAGACGATATATCTTATGGAATTGCTATGGCACAAGAGGTGTTAGGTA
TGGTTTTAGCAGATTATATTGAGCATGAAGATCTACTTCCAGAACCAACACCAATCAATAAGATTTCTGTTGAAGATGAT
TCTTTTACTACTTTAATTCGTGTAGATGTTGCCAAATATTTGAAAGACACAGAACTTGTCAAAAAAACCTTAACAATTCC
TCAATGGGCGGATAAGCTAGGCAAAAGGGCTGGCATAAATTTTTCAGTCCTTCTAACAGAATCAATTGCAGATAAAGCAG
ATAACATTCTTCGTTCTGGAAGAAATAATTAG

Structures

Antitoxin

• PDB: 5ZGN
• AlphaFold DB: A0A0H3GLZ1