Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3996144..3996763 Replicon chromosome
Accession NZ_CP056530
Organism Klebsiella pneumoniae strain RHBSTW-00433

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag HV193_RS19530 Protein ID WP_002892050.1
Coordinates 3996545..3996763 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag HV193_RS19525 Protein ID WP_002892066.1
Coordinates 3996144..3996518 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HV193_RS19515 3991411..3994557 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
HV193_RS19520 3994687..3995721 + 1035 Protein_3826 IS481-like element ISKpn28 family transposase -
HV193_RS19525 3996144..3996518 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
HV193_RS19530 3996545..3996763 + 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
HV193_RS19535 3996922..3997488 + 567 WP_002892030.1 maltose O-acetyltransferase -
HV193_RS19540 3997460..3997600 - 141 WP_004147370.1 hypothetical protein -
HV193_RS19545 3997621..3998091 + 471 WP_002892026.1 YlaC family protein -
HV193_RS19550 3998066..3999517 - 1452 WP_040236504.1 PLP-dependent aminotransferase family protein -
HV193_RS19555 3999618..4000316 + 699 WP_181469495.1 GNAT family N-acetyltransferase -
HV193_RS19560 4000313..4000453 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
HV193_RS19565 4000453..4000716 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 3994687..3995985 1298


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T163630 WP_002892050.1 NZ_CP056530:3996545-3996763 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T163630 NZ_CP072708:18472-18729 [Enterococcus faecium]
ATGATTAAGGCTTGGTCTGATGATGCTTGGGATGATTATCTTTATTGGCATGAGCAAGGAAACAAAAGCAATATAAAAAA
GATTAACAAGTTAATAAAAGATATCGATCGTTCCCCCTTTGCTGGATTAGGAAAACCTGAGCCATTAAAGCATGATTTAT
CTGGAAAATGGTCCAGAAGAATTACAGATGAACATAGACTGATATATAGAGTTGAAAATGAAACGATATTTATTTATTCT
GCAAAAGATCACTATTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT163630 WP_002892066.1 NZ_CP056530:3996144-3996518 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT163630 NZ_CP072708:18210-18479 [Enterococcus faecium]
ATGGAAGCAGTAGCTTATTCAAATTTCCGCCAAAATTTACGTAGTTATATGAAACAAGTTAATGAGGATGCTGAAACACT
TATTGTAACAAGTAAAGATGTAGAAGATACAGTTGTTGTATTATCAAAAAGAGATTATGATTCTATGCAAGAAACGTTGA
GAACACTTTCTAATAATTACGTCATGGAAAAAATTCGTCGAGGAGATGAACAATTCTCCAAAGGTGCATTTAAAACACAT
GACTTAATCGAGGTTGAATCTGATGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References