Detailed information of TA system TA163615

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-TIGR02293
• Location: 147889..148790
• Replicon: plasmid pRHBSTW-00444_3
• Accession: NZ_CP056517
• Organism: Citrobacter freundii strain RHBSTW-00444

Toxin (Protein)

• Gene name: -
• Uniprot ID: A0A837LJP8
• Locus tag: HV198_RS25365
• Protein ID: WP_007372300.1
• Coordinates: 147889..148347 (-)
• Length: 153 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: HV198_RS25370
• Protein ID: WP_008786588.1
• Coordinates: 148344..148790 (-)
• Length: 149 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV198_RS25320	143615..143857	+	243	WP_007372291.1	hypothetical protein	-
HV198_RS25325	143868..144266	+	399	WP_007372292.1	hypothetical protein	-
HV198_RS25330	144350..144676	+	327	WP_007372293.1	hypothetical protein	-
HV198_RS25335	145039..145377	-	339	WP_007372294.1	hypothetical protein	-
HV198_RS25340	145615..146058	+	444	WP_007372295.1	hypothetical protein	-
HV198_RS25345	146177..146518	+	342	WP_007372296.1	hypothetical protein	-
HV198_RS25350	146677..147231	+	555	WP_007372297.1	hypothetical protein	-
HV198_RS25355	147281..147547	+	267	WP_007372298.1	hypothetical protein	-
HV198_RS25360	147615..147845	-	231	WP_007372299.1	hypothetical protein	-
HV198_RS25365	147889..148347	-	459	WP_007372300.1	RES family NAD+ phosphorylase	Toxin
HV198_RS25370	148344..148790	-	447	WP_008786588.1	DUF2384 domain-containing protein	Antitoxin
HV198_RS25375	148939..149256	+	318	WP_007372302.1	hypothetical protein	-
HV198_RS25380	149298..150140	+	843	WP_007372303.1	hypothetical protein	-
HV198_RS25385	150112..150390	+	279	WP_007372304.1	hypothetical protein	-
HV198_RS25390	150450..150893	+	444	WP_007372305.1	hypothetical protein	-
HV198_RS25395	151180..151344	+	165	WP_007372306.1	hypothetical protein	-
HV198_RS25400	151912..152754	+	843	WP_007372307.1	HNH endonuclease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	blaSHV-12	-	1..183652	183652

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (3-148)

Antitoxin

• Antitoxin Xre/MbcA/ParS-like, toxin-binding domain (95-145)

Sequences

Toxin

Protein

• Length: 153 a.a.
• Molecular weight: 16975.40 Da
• Isoelectric Point: 4.5730

>T163615 WP_007372300.1 NZ_CP056517:c148347-147889 [Citrobacter freundii]
VILYRLTKTRYLMTAWSGLGAKEAGGRWNSVGTAMVYLSETASLTMLETLVHIHAPQLLDDFTLLSLDVPDDQIQTFDMS
RLPDNWASEDAPAELALYGDNWAESGSSIALRIPSALSPVEFNYLLNPAHPELFDLMATVKKIPFRFDSRLK

Nucleotide

• Length: 459 bp

>T163615 NZ_CP072689:1949795-1949898 [Escherichia marmotae]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 149 a.a.
• Molecular weight: 16689.02 Da
• Isoelectric Point: 6.9812

>AT163615 WP_008786588.1 NZ_CP056517:c148790-148344 [Citrobacter freundii]
MTMKVFSPDVTKTNQHCLWQVAGLDNDGIALMDKINHGLDGTVAKHISEWANITPSELRKMSGIPNTTFNRSIKDRFTAD
QSERLVRIIRVIERAVELFEGDKEAAHKWLNEANRGLSWKSPAELVSSETGALEVMRLITRIEHGVYS

Nucleotide

• Length: 447 bp

>AT163615 NZ_CP072689:c1949912-1949667 [Escherichia marmotae]
CTGGCTTTATAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCG
CTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCTTTTAAGAATAGATGACGACGCCAGGTTTTCCAGTCCGCGCG
TAAAGTGGTCAGAAAAAAAGCGCGTTTATCATCCTCTAAAATGTTAAAAACCGCCCATTCTGGTGAAAGCACCCGGGCGG
TTTTTT

Structures

Toxin

• AlphaFold DB: A0A837LJP8