Detailed information of TA system TA163614

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 140590..141140
• Replicon: plasmid pRHBSTW-00444_3
• Accession: NZ_CP056517
• Organism: Citrobacter freundii strain RHBSTW-00444

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: S1EZ93
• Locus tag: HV198_RS25295
• Protein ID: WP_007372286.1
• Coordinates: 140832..141140 (+)
• Length: 103 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: S1FMC3
• Locus tag: HV198_RS25290
• Protein ID: WP_007372285.1
• Coordinates: 140590..140829 (+)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV198_RS25245	135597..135992	+	396	WP_024196081.1	hypothetical protein	-
HV198_RS25250	136174..136365	+	192	WP_016241560.1	hypothetical protein	-
HV198_RS25255	136496..137191	+	696	WP_007372278.1	hypothetical protein	-
HV198_RS25260	137214..137411	+	198	WP_032155220.1	Lar family restriction alleviation protein	-
HV198_RS25265	137464..137922	+	459	WP_008786599.1	hypothetical protein	-
HV198_RS25270	138070..138210	+	141	WP_007372281.1	hypothetical protein	-
HV198_RS25275	138226..138858	+	633	WP_007372282.1	hypothetical protein	-
HV198_RS25280	139319..139990	+	672	WP_007372283.1	hypothetical protein	-
HV198_RS25285	139987..140451	+	465	WP_008786596.1	hypothetical protein	-
HV198_RS25290	140590..140829	+	240	WP_007372285.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
HV198_RS25295	140832..141140	+	309	WP_007372286.1	CcdB family protein	Toxin
HV198_RS25300	141161..141319	+	159	WP_016241558.1	hypothetical protein	-
HV198_RS25305	141474..142028	+	555	WP_007372288.1	hypothetical protein	-
HV198_RS25310	142098..142715	+	618	WP_007372289.1	hypothetical protein	-
HV198_RS25315	142798..143268	+	471	WP_007372290.1	hypothetical protein	-
HV198_RS25320	143615..143857	+	243	WP_007372291.1	hypothetical protein	-
HV198_RS25325	143868..144266	+	399	WP_007372292.1	hypothetical protein	-
HV198_RS25330	144350..144676	+	327	WP_007372293.1	hypothetical protein	-
HV198_RS25335	145039..145377	-	339	WP_007372294.1	hypothetical protein	-
HV198_RS25340	145615..146058	+	444	WP_007372295.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	blaSHV-12	-	1..183652	183652

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-101)

Antitoxin

• Post-segregation antitoxin CcdA (9-79)

Sequences

Toxin

Protein

• Length: 103 a.a.
• Molecular weight: 11351.17 Da
• Isoelectric Point: 8.5044

>T163614 WP_007372286.1 NZ_CP056517:140832-141140 [Citrobacter freundii]
MQYTVYRNPGNSQAYPYLLDIQSDIIGELNTRLVIPLHRLKKGASAPVARLTPVIQVEGNDVILMTHEMASVRVKQLGQA
VMDASPFRHTIKSAVDFLLDGF

Nucleotide

• Length: 309 bp

>T163614 NZ_CP072689:c1155890-1155672 [Escherichia marmotae]
ATGTCCGATAAACCTTTAACAAAAACCGATTATTTGATGCGTTTGCGTCGTTGTCAGACAATTGACACGCTGGAACGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTTGCAGAAT
TGACCATGAATAAGCTTTACGACAAGATCCCTTCCTCGGTGTGGAAATTTATTCGATAA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 8784.97 Da
• Isoelectric Point: 8.6387

>AT163614 WP_007372285.1 NZ_CP056517:140590-140829 [Citrobacter freundii]
MQATAARQKKTVSVTLEPMLVQQAKDAGINLSATLAEALQSKLKASAAEEWKRQNRAGIQELNRITEEHGLLSDEYRTF

Nucleotide

• Length: 240 bp

>AT163614 NZ_CP072689:c1156290-1155895 [Escherichia marmotae]
ATGGATGAATACTCACCAAAAAGACATGATATTGCACAGCTTAAGTTTCTTTGCGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGTTGGGTTAACGACCCTACCTCGGCTATCAATCTGCAACTTAATGAACTCATTGAGC
ATATTGCAACCTTCGCGCTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATAGATGAATATCTG
GACGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTCCAGAAATGGCGGAAGTCAGGTAACCGACT
GTTCCGTTGTTTCGTGAATGCCACGAAAGAGAATCCTGCAAGTTTATCTTGTTCGAATTATTACAACCATGGGTAG

Structures

Toxin

• AlphaFold DB: S1EZ93

Antitoxin

• AlphaFold DB: S1FMC3