Detailed information of TA system TA163613

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): kacAT/ElaA-DUF1778
• Location: 160451..161248
• Replicon: plasmid pRHBSTW-00444_2
• Accession: NZ_CP056516
• Organism: Citrobacter freundii strain RHBSTW-00444

Toxin (Protein)

• Gene name: KacT
• Uniprot ID: -
• Locus tag: HV198_RS24120
• Protein ID: WP_171279078.1
• Coordinates: 160745..161248 (+)
• Length: 168 a.a.

Antitoxin (Protein)

• Gene name: KacA
• Uniprot ID: A0A5D4NAG3
• Locus tag: HV198_RS24115
• Protein ID: WP_016582626.1
• Coordinates: 160451..160720 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV198_RS24075	155609..155884	-	276	WP_000732275.1	mercury resistance system periplasmic binding protein MerP	-
HV198_RS24080	155900..156310	-	411	WP_020833727.1	mercuric ion transporter MerT	-
HV198_RS24085	156382..156837	+	456	WP_001166628.1	Hg(II)-responsive transcriptional regulator	-
HV198_RS24090	157092..157850	-	759	WP_171279050.1	DUF2971 domain-containing protein	-
HV198_RS24095	157960..158370	-	411	WP_060458854.1	hypothetical protein	-
HV198_RS24100	158360..159004	-	645	WP_060458853.1	hypothetical protein	-
HV198_RS24105	159006..159359	-	354	WP_171279077.1	hypothetical protein	-
HV198_RS24110	159365..160030	-	666	WP_000161333.1	AAA family ATPase	-
HV198_RS24115	160451..160720	+	270	WP_016582626.1	DUF1778 domain-containing protein	Antitoxin
HV198_RS24120	160745..161248	+	504	WP_171279078.1	GNAT family N-acetyltransferase	Toxin
HV198_RS24125	161277..161483	-	207	WP_171279051.1	hypothetical protein	-
HV198_RS24130	161434..161685	-	252	WP_000147960.1	hypothetical protein	-
HV198_RS24135	161687..162379	-	693	WP_171279052.1	hypothetical protein	-
HV198_RS24140	162393..162716	-	324	WP_000064173.1	hypothetical protein	-
HV198_RS24145	162903..163487	-	585	WP_181507064.1	tail fiber assembly protein	-
HV198_RS24150	163501..165882	-	2382	WP_181507079.1	phage tail protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	aph(3'')-Ib / aph(6)-Id	-	1..216816	216816

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (31-142)

Antitoxin

• Vibrio phage ICP1, Orf50 (8-86)

Sequences

Toxin

Protein

• Length: 168 a.a.
• Molecular weight: 18612.31 Da
• Isoelectric Point: 8.8885

>T163613 WP_171279078.1 NZ_CP056516:160745-161248 [Citrobacter freundii]
MFSAQAVYDFSGFDCGEPSLNDFLQNRLVHQHNGRILRGYLLLTKDPVPKVKGFYTLSGSCFARQTLPSNTQKRKIPYAD
APSVTLGRLAIDISLQKQGYGEVLVTHALKVVYQASRAVGIYALFVDALNQSAMQFYQKFGFIPLTGANANSLFYPTKSI
EELFETE

Nucleotide

• Length: 504 bp

>T163613 NZ_CP072689:c699036-698884 [Escherichia marmotae]
ATGAAGCAGCATAAGGCGATGATTGTCGCCCTGATCGTCATCTGTATCACCGCCGTAGTGGCGGCGCTGGTAACGAGAAA
AGACCTCTGTGAGGTTCACATCCGAACTGGCCAGACGGAGGTTGCTGTTTTCACGGCTTACGAATCCGAGTAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10113.57 Da
• Isoelectric Point: 5.8580

>AT163613 WP_016582626.1 NZ_CP056516:160451-160720 [Citrobacter freundii]
MSALKKQRIDLRLTDDDKSMIEEAAAMTNQTITQFMVASASERAAEVIEQHRRLILSEESWNLVMDAISNPPAPNDRLKR
AAKRLQSME

Nucleotide

• Length: 270 bp

>AT163613 NZ_CP072689:699085-699142 [Escherichia marmotae]
GTTCAGCATATAGGGGGCCTCGGGTTGATGGTAAAATATCACTCGGGGCTTTTCTCTA

Structures

Antitoxin

• AlphaFold DB: A0A5D4NAG3