Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 71855..72498 Replicon plasmid pRHBSTW-00444_2
Accession NZ_CP056516
Organism Citrobacter freundii strain RHBSTW-00444

Toxin (Protein)


Gene name vagD Uniprot ID D7Y5A6
Locus tag HV198_RS23610 Protein ID WP_001044765.1
Coordinates 72082..72498 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag HV198_RS23605 Protein ID WP_001261282.1
Coordinates 71855..72085 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HV198_RS23580 67368..67634 - 267 WP_024144810.1 type II toxin-antitoxin system ParD family antitoxin -
HV198_RS23585 67865..69538 - 1674 WP_000026497.1 hypothetical protein -
HV198_RS23590 70103..70912 - 810 WP_000654269.1 hypothetical protein -
HV198_RS23595 70930..71292 - 363 WP_000533745.1 hypothetical protein -
HV198_RS23600 71476..71898 - 423 WP_162534719.1 hypothetical protein -
HV198_RS23605 71855..72085 + 231 WP_001261282.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
HV198_RS23610 72082..72498 + 417 WP_001044765.1 type II toxin-antitoxin system VapC family toxin Toxin
HV198_RS23615 72572..74134 + 1563 WP_004206609.1 AAA family ATPase -
HV198_RS23620 74119..75141 + 1023 WP_000361404.1 helicase UvrD -
HV198_RS23630 76012..76569 - 558 WP_003100847.1 recombinase family protein -
HV198_RS23635 76563..76934 - 372 WP_003100853.1 hypothetical protein -
HV198_RS23640 76931..77431 - 501 WP_003100856.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid aph(3'')-Ib / aph(6)-Id - 1..216816 216816


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15148.60 Da        Isoelectric Point: 7.1082

>T163611 WP_001044765.1 NZ_CP056516:72082-72498 [Citrobacter freundii]
VNKTYMLDTCICSFIMREQPEAVLKRLELAVLRGQRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPDLVLEDWVR

Download         Length: 417 bp

>T163611 NZ_CP072689:c390648-390271 [Escherichia marmotae]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCACGCCCGTCTCCGGTTGAAATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCATTACGGCCTTACGCTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTACGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGTTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCACGCCGGGCAACCGGCCTGATGACACGCGA
CAACTACAGAACGGTAAATAACATTACCCTGGGTAAACATCCGGGGGCGAAACAATGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT163611 WP_001261282.1 NZ_CP056516:71855-72085 [Citrobacter freundii]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT163611 NZ_CP072689:c391106-390738 [Escherichia marmotae]
GTGTCAGACACATTCTCCGGGACAACGCATCCCGATGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGTACCGTGAC
ACCTTGTTTTGGCGCCCGTCTGGTGCAGGAGGGCAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATTCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATTCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCAGTTG
TGGCTACGTTTATATGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2H9AY90

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References