Detailed information of TA system TA163609

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 4216121..4216648
• Replicon: chromosome
• Accession: NZ_CP056515
• Organism: Citrobacter freundii strain RHBSTW-00444

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: HV198_RS19900
• Protein ID: WP_181506235.1
• Coordinates: 4216121..4216396 (-)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: HV198_RS19905
• Protein ID: WP_094760973.1
• Coordinates: 4216400..4216648 (-)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV198_RS19875	4211178..4212119	+	942	WP_181506231.1	4-hydroxyproline epimerase	-
HV198_RS19880	4212116..4213219	+	1104	WP_181506232.1	FAD-binding oxidoreductase	-
HV198_RS19885	4213216..4213470	+	255	WP_146717592.1	(2Fe-2S)-binding protein	-
HV198_RS19890	4213457..4214713	+	1257	WP_181506233.1	NAD(P)/FAD-dependent oxidoreductase	-
HV198_RS19895	4215209..4216006	-	798	WP_181506234.1	helix-turn-helix transcriptional regulator	-
HV198_RS19900	4216121..4216396	-	276	WP_181506235.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HV198_RS19905	4216400..4216648	-	249	WP_094760973.1	ribbon-helix-helix domain-containing protein	Antitoxin
HV198_RS19910	4216725..4217558	-	834	WP_181506236.1	DUF4942 domain-containing protein	-
HV198_RS19915	4217674..4218015	-	342	WP_181506237.1	TA system toxin CbtA family protein	-
HV198_RS19920	4218071..4218304	-	234	WP_181506238.1	hypothetical protein	-
HV198_RS19925	4218301..4218654	-	354	WP_181506239.1	type IV toxin-antitoxin system YeeU family antitoxin	-
HV198_RS19930	4218700..4218921	-	222	WP_181506240.1	DUF987 family protein	-
HV198_RS19935	4218918..4219427	-	510	WP_181506241.1	DNA repair protein RadC	-
HV198_RS19940	4219440..4219859	-	420	WP_181507050.1	antirestriction protein	-
HV198_RS19945	4219975..4220793	-	819	WP_181506242.1	DUF945 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (9-42)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10928.36 Da
• Isoelectric Point: 5.1406

>T163609 WP_181506235.1 NZ_CP056515:c4216396-4216121 [Citrobacter freundii]
MEIYWTLKAQDDLERIYRFALQYSRQPADNVLDRLIIGCAGLTAHPAIGVQQTRYEPREVRKVLFDDYEVHYELRGNDIY
IVDLWHTKEER

Nucleotide

• Length: 276 bp

>T163609 NZ_CP072688:c4586354-4586181 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTTCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 8987.33 Da
• Isoelectric Point: 8.8294

>AT163609 WP_094760973.1 NZ_CP056515:c4216648-4216400 [Citrobacter freundii]
MGSLTKQTISAQIPVELAAAVENLAIELDRSKSWIIKEALTSMLAERERRHQSIQAGLADVDAGRVVSHSNMVDFANRLK
KA

Nucleotide

• Length: 249 bp

>AT163609 NZ_CP072688:c4586678-4586382 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAACTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAGCATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG