Detailed information of TA system TA163608

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 4178723..4179299
• Replicon: chromosome
• Accession: NZ_CP056515
• Organism: Citrobacter freundii strain RHBSTW-00444

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: -
• Locus tag: HV198_RS19735
• Protein ID: WP_117342369.1
• Coordinates: 4179012..4179299 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: -
• Locus tag: HV198_RS19730
• Protein ID: WP_117342368.1
• Coordinates: 4178723..4179025 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV198_RS19715	4175191..4177341	+	2151	WP_135912629.1	pyruvate/proton symporter BtsT	-
HV198_RS19720	4177449..4177643	+	195	WP_003830038.1	YbdD/YjiX family protein	-
HV198_RS19725	4177656..4178612	+	957	WP_181506225.1	GTPase	-
HV198_RS19730	4178723..4179025	-	303	WP_117342368.1	BrnA antitoxin family protein	Antitoxin
HV198_RS19735	4179012..4179299	-	288	WP_117342369.1	BrnT family toxin	Toxin
HV198_RS19740	4179472..4179636	-	165	WP_117342370.1	DUF1127 domain-containing protein	-
HV198_RS19745	4179813..4181225	+	1413	WP_181506226.1	PLP-dependent aminotransferase family protein	-
HV198_RS19750	4181252..4182202	-	951	WP_117342372.1	Rpn family recombination-promoting nuclease/putative transposase	-
HV198_RS19755	4182476..4183717	+	1242	WP_181506227.1	multidrug efflux MFS transporter MdtM	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (42-93)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11362.75 Da
• Isoelectric Point: 7.5239

>T163608 WP_117342369.1 NZ_CP056515:c4179299-4179012 [Citrobacter freundii]
MPMEFEWDANKAQSNHRKHGVRFEDAVLVFDDPRHLSRQDRYENGEYRWQTIGLVHGIVVILVAHSVRFESGFEVIRIIS
ARKADRKERNRYEHD

Nucleotide

• Length: 288 bp

>T163608 NZ_CP072688:4268052-4268390 [Escherichia coli]
ATGACTGACACGCATTCTATTGCACAACCGTTCGAAGCAGAAGTCTCCCCGGCAAATAACCGTCAATTAACCGTGAGTTA
TGCGAGTCGCTACCCGGATTACAACCGTATTCCCGCCATCACCCTGAAAGGTCAGTGGCTGGAAGCCGCCGGTTTTGCCA
CCGGCACGGCGGTAGATGTCAAAGTGATGGAAGGCTGTATTGTCCTCACCGCCCAACCACCCGCCGCCGAAGAGAGCGAA
CTGATGCAGTCACTGCGCCAGGTGTGCAAGCTGTCGGCGCGTAAACAAAAGCAGGTGCAGGATTTTATTGGGGTGATTAG
TAACAAAACGCCACGCTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11444.95 Da
• Isoelectric Point: 8.5366

>AT163608 WP_117342368.1 NZ_CP056515:c4179025-4178723 [Citrobacter freundii]
MSMIKHKRGSASALSAQHEAELKALANKPDDDIDYSDIPVTEDEQWSEANRGTFFRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLREQNKK

Nucleotide

• Length: 303 bp

>AT163608 NZ_CP072688:c4268057-4267981 [Escherichia coli]
AGTCATAACTGCTATTCCCTATAAATAGTGATTGTGATGAGCGGTGCGGGTGTGTTGGCGCACATCCGCACCGCGCT