Detailed information of TA system TA163596

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 837032..837810
• Replicon: chromosome
• Accession: NZ_CP056515
• Organism: Citrobacter freundii strain RHBSTW-00444

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: -
• Locus tag: HV198_RS04045
• Protein ID: WP_181506573.1
• Coordinates: 837322..837810 (+)
• Length: 163 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: -
• Locus tag: HV198_RS04040
• Protein ID: WP_181506572.1
• Coordinates: 837032..837325 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV198_RS04025	833616..834575	+	960	WP_181506571.1	SUMF1/EgtB/PvdO family nonheme iron enzyme	-
HV198_RS04030	834572..835441	+	870	Protein_790	SUMF1/EgtB/PvdO family nonheme iron enzyme	-
HV198_RS04035	835419..836708	+	1290	WP_181507012.1	type VI secretion system ImpA family N-terminal domain-containing protein	-
HV198_RS04040	837032..837325	+	294	WP_181506572.1	DUF1778 domain-containing protein	Antitoxin
HV198_RS04045	837322..837810	+	489	WP_181506573.1	GNAT family N-acetyltransferase	Toxin
HV198_RS04050	837948..838774	-	827	Protein_794	IS3 family transposase	-
HV198_RS04060	839124..839831	-	708	WP_181506574.1	DUF554 domain-containing protein	-
HV198_RS04065	840300..842435	+	2136	WP_115257507.1	ornithine decarboxylase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (42-143)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-88)

Sequences

Toxin

Protein

• Length: 163 a.a.
• Molecular weight: 17762.68 Da
• Isoelectric Point: 7.2658

>T163596 WP_181506573.1 NZ_CP056515:837322-837810 [Citrobacter freundii]
MISAPEPLHAGHILTPFCCGVDSMDNWLQQRAMKNQLSGASRTFVCCDDAKVMAYYSLASSAVTTNTASGRFRRNMPDPI
PVVVLGRLAVDQSLHGRRIGRALIRDAGLRVIQVAETIGIRGMLVHALSDEAREFYLRVGFEPSPMDPMMLMVTLGDLVE
SI

Nucleotide

• Length: 489 bp

>T163596 NZ_CP072688:1182830-1183141 [Escherichia coli]
ATGCACATCATTTCTAAAGCGCCATTTGAAGAGTCTGCGCGTAAATATCCTAACGACGCTCTGGCTCTTCAGGCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACGCCAGAAGAAATGCGAACGGCGTTTCCCAACCTCGACAATTTTAAATATC
GCAACAAATGGTACGTTCTGGATGTAGGTGGGAACAACCTAAGGGTGATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACTAATCACGCCGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10903.53 Da
• Isoelectric Point: 8.6141

>AT163596 WP_181506572.1 NZ_CP056515:837032-837325 [Citrobacter freundii]
MPAANSMAMKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPEAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEK

Nucleotide

• Length: 294 bp

>AT163596 NZ_CP072688:1183138-1183557 [Escherichia coli]
ATGACTGCTAATGCTGTACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGCAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGACGAGCCGCATTGCAGAATATGAAAACAACAACGAAAAGTTTGCAGAGTTCGACAAAGCCGTTGCGGCAATGCCTGTT
GGTGTGGCGTTACTTCGCACGCTGATTGATCAACATAATCTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGCCAGATCTTGTCTGGACAACGATCCCTGACGATATCCCACATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA