Detailed information of TA system TA163592

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 98749..99275
• Replicon: plasmid pRHBSTW-00449_2
• Accession: NZ_CP056506
• Organism: Citrobacter freundii strain RHBSTW-00449

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1EXL1
• Locus tag: HV201_RS25880
• Protein ID: WP_000323025.1
• Coordinates: 98749..99036 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: S1F6D3
• Locus tag: HV201_RS25885
• Protein ID: WP_000534858.1
• Coordinates: 99036..99275 (-)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV201_RS25850	94182..94457	+	276	WP_057064204.1	hypothetical protein	-
HV201_RS25855	94511..94867	+	357	WP_165410135.1	hypothetical protein	-
HV201_RS25860	94936..96954	+	2019	WP_181522832.1	ParB/RepB/Spo0J family partition protein	-
HV201_RS25865	96990..97424	+	435	WP_057064207.1	conjugation system SOS inhibitor PsiB	-
HV201_RS25870	97421..98143	+	723	WP_057064208.1	plasmid SOS inhibition protein A	-
HV201_RS25875	98146..98472	+	327	WP_057102321.1	hypothetical protein	-
-	98521..98645	+	125	NuclAT_0	-	-
-	98521..98645	+	125	NuclAT_0	-	-
-	98521..98645	+	125	NuclAT_0	-	-
-	98521..98645	+	125	NuclAT_0	-	-
HV201_RS25880	98749..99036	-	288	WP_000323025.1	type II toxin-antitoxin system mRNA interferase RelE	Toxin
HV201_RS25885	99036..99275	-	240	WP_000534858.1	type II toxin-antitoxin system antitoxin RelB	Antitoxin
HV201_RS25890	99538..100461	-	924	WP_000167917.1	cation diffusion facilitator family transporter	-
HV201_RS25895	100661..101233	-	573	WP_001515348.1	cytochrome b/b6 domain-containing protein	-
HV201_RS25900	101876..103216	-	1341	WP_058671058.1	ISNCY family transposase	-
HV201_RS25905	103304..103462	+	159	WP_023228551.1	type I toxin-antitoxin system Hok family toxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..142000	142000

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-86)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-79)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11225.22 Da
• Isoelectric Point: 10.1967

>T163592 WP_000323025.1 NZ_CP056506:c99036-98749 [Citrobacter freundii]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Nucleotide

• Length: 288 bp

>T163592 NZ_CP072687:4557402-4557776 [Escherichia coli]
ATGAACACATTACCCGATACACACATACGGGAGGCATCGCATTGCCAGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCATTACGGCCTCACACTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACTCGTTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCCCGCCGGGCAACCGGCCTGATGGCCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGCATCCGGAGAAACGATGA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9071.48 Da
• Isoelectric Point: 4.5230

>AT163592 WP_000534858.1 NZ_CP056506:c99275-99036 [Citrobacter freundii]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Nucleotide

• Length: 240 bp

>AT163592 NZ_CP072687:4556944-4557312 [Escherichia coli]
GTGTCAGACACTCTCCCCGGGACAACGCATCCCGACGATAACAACGACCGCCCCTGGTGGGGGCTACCCTGCACCGTGAC
GCCCTGTTTTGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCAGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATGCGTACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAAGGCTGACCTGCGAAGCCGACACCCTCGGCAGTTG
TGGCTACGTTTATATGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Toxin

• PDB: 4V7K
• PDB: 4V7J
• PDB: 4FXI
• PDB: 4FXE
• PDB: 2KC8
• PDB: 4FXH
• PDB: 2KC9

Antitoxin

• AlphaFold DB: A0A4V1CTS8