Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5030642..5031258 Replicon chromosome
Accession NZ_CP056505
Organism Citrobacter freundii strain RHBSTW-00449

Toxin (Protein)


Gene name vapC Uniprot ID -
Locus tag HV201_RS24525 Protein ID WP_061548034.1
Coordinates 5030642..5031016 (-) Length 125 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID -
Locus tag HV201_RS24530 Protein ID WP_162009218.1
Coordinates 5031016..5031258 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HV201_RS24510 5028145..5029047 + 903 WP_003028691.1 formate dehydrogenase O subunit beta -
HV201_RS24515 5029044..5029679 + 636 WP_003028686.1 formate dehydrogenase cytochrome b556 subunit -
HV201_RS24520 5029676..5030605 + 930 WP_003028685.1 formate dehydrogenase accessory protein FdhE -
HV201_RS24525 5030642..5031016 - 375 WP_061548034.1 type II toxin-antitoxin system VapC family toxin Toxin
HV201_RS24530 5031016..5031258 - 243 WP_162009218.1 ribbon-helix-helix domain-containing protein Antitoxin
HV201_RS24535 5031464..5032372 + 909 WP_003028676.1 alpha/beta hydrolase -
HV201_RS24540 5032523..5033464 - 942 WP_003825286.1 fatty acid biosynthesis protein FabY -
HV201_RS24545 5033509..5033946 - 438 WP_003840439.1 D-tyrosyl-tRNA(Tyr) deacylase -
HV201_RS24550 5033943..5034815 - 873 WP_003028669.1 virulence factor BrkB family protein -
HV201_RS24555 5034809..5035408 - 600 WP_003028663.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Ribbon-helix-helix protein, CopG

(13-47)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13648.86 Da        Isoelectric Point: 9.6927

>T163591 WP_061548034.1 NZ_CP056505:c5031016-5030642 [Citrobacter freundii]
MVKGSALFDTNILIDLFSGRNEAKLAIETWPPQNAISLITWMEVMVGAKKHHQEARTRVALGAFNVIGVSQDIAERSVSI
RQEYGMKLPDAIILATAQIHRLTLVTRNTKDFAGLSGVVTPYTL

Download         Length: 375 bp

>T163591 NZ_CP072687:4221633-4221971 [Escherichia coli]
ATGACTGACACGCATTCTATTGCACAACCGTTCGAAGCAGAAGTCTCCCCGGCAAATAACCGTCAATTAACCGTGAGTTA
TGCGAGTCGCTACCCGGATTACAACCGTATTCCCGCCATCACCCTGAAAGGTCAGTGGCTGGAAGCCGCCGGTTTTGCCA
CCGGCACGGCGGTAGATGTCAAAGTGATGGAAGGCTGTATTGTCCTCACCGCCCAACCACCCGCCGCCGAAGAGAGCGAA
CTGATGCAGTCACTGCGCCAGGTGTGCAAGCTGTCGGCGCGTAAACAAAAGCAGGTGCAGGATTTTATTGGGGTGATTAG
TAACAAAACGCCACGCTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9419.69 Da        Isoelectric Point: 4.3437

>AT163591 WP_162009218.1 NZ_CP056505:c5031258-5031016 [Citrobacter freundii]
MMAGVDMGRILLDLSDEVIKRLDDLKVQRNLPRAELLREAVEQYLERQDQAETTISRALGLWQGCEEDGVEYQRKLREEW

Download         Length: 243 bp

>AT163591 NZ_CP072687:c4221638-4221562 [Escherichia coli]
AGTCATAACTGCTATTCCCTATAAATAGTGATTGTGATGAGCGGTGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References