Detailed information of TA system TA163590

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tad-ata/COG5606-COG4679
• Location: 4877955..4878621
• Replicon: chromosome
• Accession: NZ_CP056505
• Organism: Citrobacter freundii strain RHBSTW-00449

Toxin (Protein)

• Gene name: tad
• Uniprot ID: -
• Locus tag: HV201_RS23825
• Protein ID: WP_048220009.1
• Coordinates: 4878262..4878621 (-)
• Length: 120 a.a.

Antitoxin (Protein)

• Gene name: ata
• Uniprot ID: -
• Locus tag: HV201_RS23820
• Protein ID: WP_003031625.1
• Coordinates: 4877955..4878272 (-)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV201_RS23785	4873875..4874687	+	813	WP_061547490.1	shikimate 5-dehydrogenase	-
HV201_RS23790	4874733..4875089	-	357	WP_061547489.1	hypothetical protein	-
HV201_RS23795	4875231..4875392	+	162	WP_003841416.1	phage protein	-
HV201_RS23800	4875634..4876776	+	1143	WP_040229863.1	hypothetical protein	-
HV201_RS23805	4876770..4877039	+	270	WP_040229864.1	hypothetical protein	-
HV201_RS23810	4877123..4877251	-	129	Protein_4673	IS3 family transposase	-
HV201_RS23815	4877667..4877945	+	279	WP_061547524.1	putative addiction module antidote protein	-
HV201_RS23820	4877955..4878272	-	318	WP_003031625.1	helix-turn-helix domain-containing protein	Antitoxin
HV201_RS23825	4878262..4878621	-	360	WP_048220009.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HV201_RS23830	4878835..4879524	+	690	WP_061547488.1	dipeptidase PepE	-
HV201_RS23835	4879590..4881221	-	1632	WP_003031618.1	Na/Pi cotransporter family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-106)

Antitoxin

• HigA2-like, helix-turn-helix domain (12-90)

Sequences

Toxin

Protein

• Length: 120 a.a.
• Molecular weight: 13491.50 Da
• Isoelectric Point: 10.1224

>T163590 WP_048220009.1 NZ_CP056505:c4878621-4878262 [Citrobacter freundii]
MTKPLYWVGHAWKDLQGMPEHVRDTFGFALWLAQQGKQHSQTKPLKGFGGAGVLEVVEDYHGNTWRAVYTIQLKNAVYVL
HVFQKKSVAGKATPKPEIDLIYQRLKAAQRHAQESGYVT

Nucleotide

• Length: 360 bp

>T163590 NZ_CP072687:4143653-4143805 [Escherichia coli]
ATGAAGCAGCATAAGGCGATGATTGTCGCCCTGATCGTCATCTGTATCACCGCCGTAGTGGCGGCGCTGGTAACGAGAAA
AGACCTCTGTGAGGTTCACATCCGAACTGGCCAGACGGAGGTTGCTGTTTTCACGGCTTACGAATCCGAGTAA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11522.46 Da
• Isoelectric Point: 9.0005

>AT163590 WP_003031625.1 NZ_CP056505:c4878272-4877955 [Citrobacter freundii]
MSHEVEASSGNVYADLGVYDAEQMQLKAQLAMVIGEILQRKGLTQQQSAQLLGMTQPKLSNLLRGQFRGISEVKMLECLV
RLGRDVEIVVGKEGNMSGKLKVKFG

Nucleotide

• Length: 318 bp

>AT163590 NZ_CP072687:c4143604-4143547 [Escherichia coli]
GTTCTGCATATAGGGGGCCTCGGGTTGATGGTAAAATATCACTCGGGGCTTTTCTCTA