Detailed information of TA system TA163589

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mosAT/AbiEii-AbiEi
• Location: 4522301..4523984
• Replicon: chromosome
• Accession: NZ_CP056505
• Organism: Citrobacter freundii strain RHBSTW-00449

Toxin (Protein)

• Gene name: mosT
• Uniprot ID: -
• Locus tag: HV201_RS22145
• Protein ID: WP_061548146.1
• Coordinates: 4523067..4523984 (+)
• Length: 306 a.a.

Antitoxin (Protein)

• Gene name: mosA
• Uniprot ID: -
• Locus tag: HV201_RS22140
• Protein ID: WP_061548147.1
• Coordinates: 4522301..4523074 (+)
• Length: 258 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV201_RS22110	4517668..4518318	+	651	WP_061548153.1	TIGR03759 family integrating conjugative element protein	-
HV201_RS22115	4518294..4518824	+	531	WP_061548152.1	transglycosylase SLT domain-containing protein	-
HV201_RS22120	4518821..4519228	+	408	WP_061548151.1	integrating conjugative element protein	-
HV201_RS22125	4519225..4521258	+	2034	WP_061548150.1	type IV conjugative transfer system coupling protein TraD	-
HV201_RS22130	4521221..4521487	+	267	WP_061548149.1	hypothetical protein	-
HV201_RS22135	4521487..4522170	+	684	WP_061548148.1	TIGR03747 family integrating conjugative element membrane protein	-
HV201_RS22140	4522301..4523074	+	774	WP_061548147.1	type IV toxin-antitoxin system AbiEi family antitoxin	Antitoxin
HV201_RS22145	4523067..4523984	+	918	WP_061548146.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
HV201_RS22150	4524023..4524316	+	294	WP_082804811.1	DUF4400 domain-containing protein	-
HV201_RS22155	4524396..4526126	+	1731	WP_181522659.1	hypothetical protein	-
HV201_RS22160	4526214..4526531	+	318	WP_061548143.1	conjugal transfer protein	-
HV201_RS22165	4526528..4526761	+	234	WP_049059556.1	TIGR03758 family integrating conjugative element protein	-
HV201_RS22170	4526780..4527106	+	327	WP_044059507.1	TIGR03745 family integrating conjugative element membrane protein	-
HV201_RS22175	4527110..4527463	+	354	WP_061548142.1	TIGR03750 family conjugal transfer protein	-
HV201_RS22180	4527460..4528086	+	627	WP_061548141.1	TIGR03746 family integrating conjugative element protein	-
HV201_RS22185	4528083..4528865	+	783	WP_061548140.1	TIGR03749 family integrating conjugative element protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	4502509..4571426	68917

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (13-278)

Antitoxin

• Transcriptional regulator, AbiEi 3 antitoxin, Type IV TA system (95-246)

Sequences

Toxin

Protein

• Length: 306 a.a.
• Molecular weight: 34708.76 Da
• Isoelectric Point: 4.8346

>T163589 WP_061548146.1 NZ_CP056505:4523067-4523984 [Citrobacter freundii]
MDRTSPYYRQVALLVRALPYVANEPCFALKGGTAINLFIRDFPRLSVDIDLAYIPLESREVALPNVREALTRIAAELEAQ
AGIHAVLQTHNPDEMRIIVTTDDAQIKIEVSPVARGTLYPPQERDVVEAVEEEFGFAAIPVVSLPDLYGGKLCAALDRQH
PRDFYDVKMLLDTQGIDRAIFNGFIAYLLSHNRPLSEVLSPRWKDITEPFQNEFSGMTFEEVSLEALREVPERMLSALKA
HFTQQDFDFLMSFKRGEPDWNLAPEPHIQNLPAVQWKLRNIGRMSEGKRAQSLEKLEKVMTEWLS

Nucleotide

• Length: 918 bp

>T163589 NZ_CP072687:c4106707-4106393 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCGTTTCCCTTTGTTATTGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCCGTATCGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGGTAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin

Protein

• Length: 258 a.a.
• Molecular weight: 29245.67 Da
• Isoelectric Point: 9.9460

>AT163589 WP_061548147.1 NZ_CP056505:4522301-4523074 [Citrobacter freundii]
MASKINWLLQNTAPGSLVLQSWLTRNNISPSLAFKYAQNGWLIKLRAGVYARAGREPDWSDALWCLQSQLEAPVHLAGLS
SLSWQGRSHYLQLKQNQCWLSVENKAILPKWFKEFPGVEWLVVSALKLPELDEKYRTELEVKGKRITASTPELAAYELLS
AVPQEISFEHAAELFQGLVNLNPRKVEKLLTLSQSVQTKRLYLFFASFYEHAWLKRVDTSKIYLGSGNRQVVVNGKLNTQ
YQITVPENFTKKGISHG

Nucleotide

• Length: 774 bp

>AT163589 NZ_CP072687:c4106943-4106710 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG