Detailed information of TA system TA163588

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): rnlAB/RnlA-RnlB
• Location: 4512292..4513732
• Replicon: chromosome
• Accession: NZ_CP056505
• Organism: Citrobacter freundii strain RHBSTW-00449

Toxin (Protein)

• Gene name: rnlA
• Uniprot ID: -
• Locus tag: HV201_RS22070
• Protein ID: WP_181522655.1
• Coordinates: 4512292..4513368 (+)
• Length: 359 a.a.

Antitoxin (Protein)

• Gene name: rnlB
• Uniprot ID: -
• Locus tag: HV201_RS22075
• Protein ID: WP_181522656.1
• Coordinates: 4513361..4513732 (+)
• Length: 124 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV201_RS22050	4508472..4509143	-	672	WP_000863683.1	hypothetical protein	-
HV201_RS22055	4509270..4510598	-	1329	WP_061548423.1	type II toxin-antitoxin system HipA family toxin	-
HV201_RS22060	4510603..4510908	-	306	WP_072291329.1	helix-turn-helix transcriptional regulator	-
HV201_RS22065	4511459..4512108	+	650	Protein_4333	AIDA repeat-containing protein	-
HV201_RS22070	4512292..4513368	+	1077	WP_181522655.1	type II toxin-antitoxin system RnlA family toxin	Toxin
HV201_RS22075	4513361..4513732	+	372	WP_181522656.1	type II toxin-antitoxin system RnlB family antitoxin	Antitoxin
HV201_RS22080	4514308..4515006	+	699	WP_181522657.1	TIGR03761 family integrating conjugative element protein	-
HV201_RS22085	4514996..4515490	+	495	WP_181522658.1	DUF3158 family protein	-
HV201_RS22090	4515539..4515814	-	276	WP_061548156.1	hypothetical protein	-
HV201_RS22095	4516056..4516472	+	417	WP_001291902.1	DUF3577 domain-containing protein	-
HV201_RS22100	4516593..4517060	+	468	WP_061548155.1	response regulator	-
HV201_RS22105	4517053..4517661	+	609	WP_061548154.1	hypothetical protein	-
HV201_RS22110	4517668..4518318	+	651	WP_061548153.1	TIGR03759 family integrating conjugative element protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	4502509..4571426	68917

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Bacterial toxin, RNase RnlA/LsoA, DBD domain (203-326)

Antitoxin

• Antitoxin RnlB/LsoB (14-111)

Sequences

Toxin

Protein

• Length: 359 a.a.
• Molecular weight: 40505.07 Da
• Isoelectric Point: 5.7993

>T163588 WP_181522655.1 NZ_CP056505:4512292-4513368 [Citrobacter freundii]
MDNRNYRNLNLDREIIETESRQFIENNNYSVHTIGDIPGGRNGLRVVFAKPGHNLATVDILYNQGGTTTVQYLTGANRDL
GKELADHLYDTINPAEFEQVNMVLQGFVEADVLSVLELSAEETHIQFSEYSRNAHSIVWKINSISFQDELTVTLHPTTGK
LHIQGRPLSCYRVFTFNLADLLDLQGLEKVLIRQDDGNANIVQQEVARAHLECVMGNAYPILSRVVEKLLVSGLCVKLAA
PDLPDYCMLLYPELRSIEGVLKQRMNNFGMLVQQDGFGPYFDKDRFSGRYSLKPQFASTLRADQANCITVAYNFYNKERH
SLFHMETAVDTSRVISDMGRLMGIATKAWVIIKDLYSV

Nucleotide

• Length: 1077 bp

>T163588 NZ_CP072687:3664010-3664228 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 124 a.a.
• Molecular weight: 13764.75 Da
• Isoelectric Point: 4.3625

>AT163588 WP_181522656.1 NZ_CP056505:4513361-4513732 [Citrobacter freundii]
VFEITSVNVSGSLKAVVMATSYENPLSSVSEIETRLSALLGSNETGEILFDLLCANGPEWNRFVSLEMKYGRIMLDTAKI
IDEHEIPKQILNKLIFALCNHPEYLEDSVLSPEDVRHIQSLNL

Nucleotide

• Length: 372 bp

>AT163588 NZ_CP072687:3663610-3663984 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG