Detailed information of TA system TA163586

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 4464780..4465356
• Replicon: chromosome
• Accession: NZ_CP056505
• Organism: Citrobacter freundii strain RHBSTW-00449

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: -
• Locus tag: HV201_RS21840
• Protein ID: WP_003019170.1
• Coordinates: 4465069..4465356 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: -
• Locus tag: HV201_RS21835
• Protein ID: WP_003019167.1
• Coordinates: 4464780..4465082 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV201_RS21820	4461292..4463442	+	2151	WP_048219778.1	pyruvate/proton symporter BtsT	-
HV201_RS21825	4463553..4463747	+	195	WP_003830038.1	YbdD/YjiX family protein	-
HV201_RS21830	4463760..4464716	+	957	WP_003019163.1	GTPase	-
HV201_RS21835	4464780..4465082	-	303	WP_003019167.1	BrnA antitoxin family protein	Antitoxin
HV201_RS21840	4465069..4465356	-	288	WP_003019170.1	BrnT family toxin	Toxin
HV201_RS21845	4465509..4466573	-	1065	WP_048219780.1	DUF2955 domain-containing protein	-
HV201_RS21850	4466563..4467630	-	1068	WP_032948464.1	HlyD family secretion protein	-
HV201_RS21855	4467637..4468101	-	465	WP_003019178.1	MarR family transcriptional regulator	-
HV201_RS21860	4468249..4468413	-	165	WP_003019180.1	DUF1127 domain-containing protein	-
HV201_RS21865	4468591..4470003	+	1413	WP_003019183.1	PLP-dependent aminotransferase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (39-93)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11157.53 Da
• Isoelectric Point: 7.0746

>T163586 WP_003019170.1 NZ_CP056505:c4465356-4465069 [Citrobacter freundii]
MPMEFEWDANKAQSNLRKHGVRFEDAVLVFDDPQHLSRQDRHENGEYRWQTIGLVHGIVVILVAHSVRFESGFEVIRIIS
ARKADSKERNRYEHG

Nucleotide

• Length: 288 bp

>T163586 NZ_CP072687:2765508-2765615 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11462.06 Da
• Isoelectric Point: 10.1293

>AT163586 WP_003019167.1 NZ_CP056505:c4465082-4464780 [Citrobacter freundii]
MSMVKHKRGSVSALSAQHEAELKALANKSDDDIDYSDIPVTEDKQWSEATRGKFFRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLREQNKK

Nucleotide

• Length: 303 bp

>AT163586 NZ_CP072687:c2765455-2765394 [Escherichia coli]
TGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT