Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yafW-ykfI/CbtA-CbeA
Location 4091496..4092175 Replicon chromosome
Accession NZ_CP056505
Organism Citrobacter freundii strain RHBSTW-00449

Toxin (Protein)


Gene name ykfI Uniprot ID A0A1B7JLK7
Locus tag HV201_RS20025 Protein ID WP_003031349.1
Coordinates 4091834..4092175 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name yafW Uniprot ID A0A1B7JLJ2
Locus tag HV201_RS20020 Protein ID WP_003031347.1
Coordinates 4091496..4091813 (+) Length 106 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HV201_RS19995 4087589..4089586 - 1998 WP_032939120.1 choline BCCT transporter BetT -
HV201_RS20000 4090090..4090281 + 192 Protein_3930 DUF905 family protein -
HV201_RS20005 4090295..4090756 + 462 WP_003031344.1 antirestriction protein -
HV201_RS20010 4090772..4091248 + 477 WP_003031345.1 RadC family protein -
HV201_RS20015 4091257..4091478 + 222 WP_003031346.1 DUF987 domain-containing protein -
HV201_RS20020 4091496..4091813 + 318 WP_003031347.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
HV201_RS20025 4091834..4092175 + 342 WP_003031349.1 type IV toxin-antitoxin system toxin YkfI Toxin
HV201_RS20030 4092797..4093342 - 546 WP_181522628.1 recombinase family protein -
HV201_RS20035 4093418..4093996 - 579 WP_128316920.1 tail fiber assembly protein -
HV201_RS20040 4093996..4095048 - 1053 WP_181522629.1 phage tail protein -
HV201_RS20045 4095048..4095728 - 681 WP_181522630.1 DUF2612 domain-containing protein -
HV201_RS20050 4095725..4096924 - 1200 WP_181522631.1 baseplate J/gp47 family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 4055134..4135032 79898


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(5-104)


Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 12921.01 Da        Isoelectric Point: 9.6543

>T163585 WP_003031349.1 NZ_CP056505:4091834-4092175 [Citrobacter freundii]
MKTLPATTQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAVDILRARQATGLLRQSRNNVVR

Download         Length: 342 bp

>T163585 NZ_CP072687:2548796-2549074 [Escherichia coli]
ATGATAATGAATTTCAGACACAAGGGATTGCGTGACTTGTTTCTTCTCGGCAAAACCTCCGGCGTTATACCGACGCAAGT
CAAACGATTACGCCACCGACTCGCTGTGATTGATGCAGCATGCTGTCTTGCTGATATCGATATGCCCGGTTACCGACTAC
ATCCGTTAAGCGGCGATCGCGATGGAATTTGGGCGATATCTGTCTCGGGCAACTGGCGAATCACATTTGAATTCGTCAAT
GGCGATGCATACATACTGGATTACGAGGACTATCACTGA

Antitoxin


Download         Length: 106 a.a.        Molecular weight: 12094.74 Da        Isoelectric Point: 7.0370

>AT163585 WP_003031347.1 NZ_CP056505:4091496-4091813 [Citrobacter freundii]
MSKITWGLQRDITPRLGARLVQEGNRLHYLADRASMTGKFSDIEYRKLDETFPHFIRQMESMLTTGELSTHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR

Download         Length: 318 bp

>AT163585 NZ_CP072687:2549074-2549358 [Escherichia coli]
ATGAAAATGGCCAATCATCCCCGCCCGGGGGACATTATTCAGGAATCACTGGACGAACTTAATGTCAGCCTGCGCGAGTT
TGCCAGAGCAATGGAAATTGCGCCCTCAACGGCAAGCCGATTGCTGACCGGAAAAGCAGCTTTGACGCCAGAAATGGCAA
TAAAACTTTCCGTGGTGATCGGCAGTTCGCCGCAAATGTGGCTGAATCTGCAAAATGCCTGGAGTCTGGCAGAGGCGGAA
AAAACGGTGGATGTGTCGCGACTGCGCCGTCTGGTTACGCAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1B7JLK7

Antitoxin

Source ID Structure
AlphaFold DB A0A1B7JLJ2

References