Detailed information of TA system TA163582

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2552528..2553167
• Replicon: chromosome
• Accession: NZ_CP056505
• Organism: Citrobacter freundii strain RHBSTW-00449

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A8B5QF36
• Locus tag: HV201_RS12405
• Protein ID: WP_003020221.1
• Coordinates: 2552528..2552704 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: HV201_RS12410
• Protein ID: WP_123924900.1
• Coordinates: 2552751..2553167 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV201_RS12385	2549306..2549536	-	231	WP_003020233.1	DUF2554 family protein	-
HV201_RS12390	2549726..2549851	-	126	WP_003020230.1	DUF2474 domain-containing protein	-
HV201_RS12395	2549851..2550861	-	1011	WP_032935681.1	cytochrome d ubiquinol oxidase subunit II	-
HV201_RS12400	2550861..2552264	-	1404	WP_061548297.1	cytochrome ubiquinol oxidase subunit I	-
HV201_RS12405	2552528..2552704	+	177	WP_003020221.1	type II toxin-antitoxin system HicA family toxin	Toxin
HV201_RS12410	2552751..2553167	+	417	WP_123924900.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
HV201_RS12415	2553260..2554669	+	1410	WP_061548296.1	PLP-dependent aminotransferase family protein	-
HV201_RS12420	2554998..2556143	+	1146	WP_061548295.1	ABC transporter substrate-binding protein	-
HV201_RS12425	2556160..2557176	+	1017	WP_061548294.1	ABC transporter ATP-binding protein	-
HV201_RS12430	2557177..2558121	+	945	WP_061548293.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-65)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6769.80 Da
• Isoelectric Point: 11.2510

>T163582 WP_003020221.1 NZ_CP056505:2552528-2552704 [Citrobacter freundii]
VKQSEFRRWLESQGVAVTNGSNHLKLRYQGRRSVMPRHPGDEIKEALRKAIIKQLDLQ

Nucleotide

• Length: 177 bp

>T163582 NZ_CP072687:c1939837-1939460 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTTCTGTC
CCGACTGCTGGACCAGCACTACGGCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAATATTATCCTGGGTAAGCACCCGGAGGTGAAACAATGA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15126.36 Da
• Isoelectric Point: 4.5716

>AT163582 WP_123924900.1 NZ_CP056505:2552751-2553167 [Citrobacter freundii]
MRYPVTLTPAVEGGFVVSFPDIPEALTQGNTRHDALQAAQAALITAFEFYFDDNEAIPLPSAVSAEDDYVEIPLSVASKV
LLLNAFLESKITQQELANRIGRPKQEITRLFDLKHTTKIDAVQIAARALGKELALTML

Nucleotide

• Length: 417 bp

>AT163582 NZ_CP072687:c1940294-1939926 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
ACAGCAATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Structures

Toxin

• AlphaFold DB: A0A8B5QF36