Detailed information of TA system TA163573

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yfjZ/CbtA-CbeA
• Location: 1630780..1631444
• Replicon: chromosome
• Accession: NZ_CP056505
• Organism: Citrobacter freundii strain RHBSTW-00449

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: -
• Locus tag: HV201_RS07775
• Protein ID: WP_180208558.1
• Coordinates: 1630780..1631106 (-)
• Length: 109 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: -
• Locus tag: HV201_RS07780
• Protein ID: WP_047363623.1
• Coordinates: 1631127..1631444 (-)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV201_RS07760	1626922..1627461	-	540	WP_003839289.1	DUF2058 domain-containing protein	-
HV201_RS07765	1627835..1629733	+	1899	WP_003845056.1	methyl-accepting chemotaxis protein	-
HV201_RS07770	1629908..1630537	+	630	WP_032936546.1	DNA-binding protein	-
HV201_RS07775	1630780..1631106	-	327	WP_180208558.1	TA system toxin CbtA family protein	Toxin
HV201_RS07780	1631127..1631444	-	318	WP_047363623.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
HV201_RS07785	1631465..1631686	-	222	WP_047363621.1	DUF987 domain-containing protein	-
HV201_RS07790	1631695..1632171	-	477	WP_000811693.1	RadC family protein	-
HV201_RS07795	1632187..1632645	-	459	WP_000211838.1	antirestriction protein	-
HV201_RS07800	1632743..1632982	-	240	WP_000194654.1	DUF905 domain-containing protein	-
HV201_RS07805	1633059..1633526	-	468	WP_001547765.1	hypothetical protein	-
HV201_RS07810	1633549..1633992	-	444	WP_001547764.1	phage transcriptional regulator	-
HV201_RS07815	1633992..1634228	-	237	WP_001144031.1	hypothetical protein	-
HV201_RS07820	1634269..1634969	-	701	Protein_1530	WYL domain-containing protein	-
HV201_RS07825	1635186..1636007	-	822	WP_047363676.1	DUF945 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-106)

Antitoxin

• Antitoxin CbeA (5-104)

Sequences

Toxin

Protein

• Length: 109 a.a.
• Molecular weight: 12347.29 Da
• Isoelectric Point: 8.0567

>T163573 WP_180208558.1 NZ_CP056505:c1631106-1630780 [Citrobacter freundii]
MKTLPATISRATKPCLSPVAVWQMLLTRLLEQHYGLTLNDTPFSEERVIQEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYIRAVDILRARQTLGLLRGSH

Nucleotide

• Length: 327 bp

>T163573 NZ_CP072686:4268234-4268572 [Escherichia coli]
ATGACTGACACGCATTCTATTGCACAACCGTTCGAAGCAGAAGTCTCCCCGGCAAATAACCGTCAATTAACCGTGAGTTA
TGCGAGTCGCTACCCGGATTACAACCGTATTCCCGCCATCACCCTGAAAGGTCAGTGGCTGGAAGCCGCCGGTTTTGCCA
CCGGCACGGCGGTAGATGTCAAAGTGATGGAAGGCTGTATTGTCCTCACCGCCCAACCACCCGCCGCCGAAGAGAGCGAA
CTGATGCAGTCACTGCGCCAGGTGTGCAAGCTGTCGGCGCGTAAACAAAAGCAGGTGCAGGATTTTATTGGGGTGATTAG
TAACAAAACGCCACGCTAA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 12130.81 Da
• Isoelectric Point: 7.8418

>AT163573 WP_047363623.1 NZ_CP056505:c1631444-1631127 [Citrobacter freundii]
MTHITWGLQRNITPRLGARLVQERNRLHYLADRASITGKFSDAEYRKLDETFPHFIRQMESMLTTAELSPQHAHCVILYH
NGFTCEADTLGSCGYVYIAVYPTQR

Nucleotide

• Length: 318 bp

>AT163573 NZ_CP072686:c4268239-4268163 [Escherichia coli]
AGTCATAACTGCTATTCCCTATAAATAGTGATTGTGATGAGCGGTGCGGGTGTGTTGGCGCACATCCGCACCGCGCT