Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 939514..940168 Replicon chromosome
Accession NZ_CP056505
Organism Citrobacter freundii strain RHBSTW-00449

Toxin (Protein)


Gene name cptA Uniprot ID A0A0J1NHY7
Locus tag HV201_RS04585 Protein ID WP_003026936.1
Coordinates 939761..940168 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID A0A6H3AT26
Locus tag HV201_RS04580 Protein ID WP_003026938.1
Coordinates 939514..939780 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HV201_RS04555 934715..936148 - 1434 WP_061547617.1 6-phospho-beta-glucosidase BglA -
HV201_RS04560 936269..936997 - 729 WP_003838265.1 MurR/RpiR family transcriptional regulator -
HV201_RS04565 937050..937361 + 312 WP_003026949.1 N(4)-acetylcytidine aminohydrolase -
HV201_RS04570 937524..938183 + 660 WP_003026947.1 hemolysin III family protein -
HV201_RS04575 938277..939257 - 981 WP_048232745.1 tRNA-modifying protein YgfZ -
HV201_RS04580 939514..939780 + 267 WP_003026938.1 FAD assembly factor SdhE Antitoxin
HV201_RS04585 939761..940168 + 408 WP_003026936.1 protein YgfX Toxin
HV201_RS04590 940213..940734 - 522 WP_003026933.1 flavodoxin FldB -
HV201_RS04595 940848..941744 + 897 WP_003026928.1 site-specific tyrosine recombinase XerD -
HV201_RS04600 941768..942481 + 714 WP_003026923.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
HV201_RS04605 942487..944220 + 1734 WP_061547616.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 15953.89 Da        Isoelectric Point: 11.4054

>T163572 WP_003026936.1 NZ_CP056505:939761-940168 [Citrobacter freundii]
VVQWQSDLRVSWRAQWLSLLIHGLVAVFILLMPWPLSYTPLWLVLLSLVVFDSVRSQRRINACQGEIRLLMDGRLRWQGQ
EWSIVSAPWMVKTGMMLRLRSDAGKRQHLWLAADSMDDAEWRDLRRLMLQKAKQK

Download         Length: 408 bp

>T163572 NZ_CP072686:c4153312-4152998 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCGTTTCCCTTTGTTATTGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCCGTATCGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGGTAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10558.09 Da        Isoelectric Point: 5.1700

>AT163572 WP_003026938.1 NZ_CP056505:939514-939780 [Citrobacter freundii]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDEEKRIFIRLLECDDPDLFNWLMNHGKPADAELEQMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT163572 NZ_CP072686:c4153548-4153315 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J1NHY7

Antitoxin

Source ID Structure
AlphaFold DB A0A6H3AT26

References