Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 4852427..4853043 Replicon chromosome
Accession NZ_CP056466
Organism Citrobacter freundii strain RHBSTW-00486

Toxin (Protein)


Gene name vapC Uniprot ID A0A0J1MQ96
Locus tag HV214_RS23490 Protein ID WP_003028682.1
Coordinates 4852427..4852801 (-) Length 125 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A6B5NTK4
Locus tag HV214_RS23495 Protein ID WP_043018956.1
Coordinates 4852801..4853043 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HV214_RS23475 4849930..4850832 + 903 WP_003028691.1 formate dehydrogenase O subunit beta -
HV214_RS23480 4850829..4851464 + 636 WP_003028686.1 formate dehydrogenase cytochrome b556 subunit -
HV214_RS23485 4851461..4852390 + 930 WP_003028685.1 formate dehydrogenase accessory protein FdhE -
HV214_RS23490 4852427..4852801 - 375 WP_003028682.1 type II toxin-antitoxin system VapC family toxin Toxin
HV214_RS23495 4852801..4853043 - 243 WP_043018956.1 ribbon-helix-helix domain-containing protein Antitoxin
HV214_RS23500 4853249..4854157 + 909 WP_181212905.1 alpha/beta hydrolase -
HV214_RS23505 4854308..4855249 - 942 WP_003825286.1 fatty acid biosynthesis protein FabY -
HV214_RS23510 4855294..4855731 - 438 WP_003028671.1 D-tyrosyl-tRNA(Tyr) deacylase -
HV214_RS23515 4855728..4856600 - 873 WP_003028669.1 virulence factor BrkB family protein -
HV214_RS23520 4856594..4857193 - 600 WP_046671351.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Ribbon-helix-helix protein, CopG

(13-47)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13674.89 Da        Isoelectric Point: 9.5622

>T163557 WP_003028682.1 NZ_CP056466:c4852801-4852427 [Citrobacter freundii]
MVKGSALFDTNILIDLFSGRNEAKLAIETWPPQNAISLITWMEVMVGAKKYHQEARTRVALGAFNVIGVSQDIAERSVSI
RQEYGMKLPDAIILATAQIHRLTLVTRNTKDFAGLSGVVTPYTL

Download         Length: 375 bp

>T163557 NZ_CP072680:c1428434-1428138 [Vibrio sp. SCSIO 43186]
ATGCAAAAGAACAAATACAAACTGAGTAAATTAGCTCAGACTCATTTACGGAAAATCAAAAGCTATACTGTTAACAACTT
CTCCGAGACCCAGTGGCGAAGCTATAAAGATACGTTACTAACTGGTTTTCAAATGCTTGCTGATAATCCAGCTGTTGGTC
GCAGTTGTGATGACATATACCCAAGCGGGTTCTATTTCCCTGTGGGCAAGCATACCGCTTATTTTACAAAAGAGGATGGC
TTCATTTTAGTTGTAGCTATACTCGGTCAATCACAGCTTCCTCAGAACCACTTATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9451.75 Da        Isoelectric Point: 4.3437

>AT163557 WP_043018956.1 NZ_CP056466:c4853043-4852801 [Citrobacter freundii]
MMAGMDMGRILLDLSDEVIKRLDDLKVQRNLPRAELLREAVEQYLERQDQAETTISRALGLWQGCEEDGVEYQRKLREEW

Download         Length: 243 bp

>AT163557 NZ_CP072680:c1428683-1428441 [Vibrio sp. SCSIO 43186]
ATGCACACATTAACAGCAAATGATGCCAAACGTAATTTTGGTGAGCTACTTCTAAGCGCCCAACGTGAGCCTGTGAAGAT
TAGCAAAAACAGTAAAGATGCCGTCGTTGTAATGTCTATCAAGGACTATGAAGAACTAGAAGCAATGAAAACCGATTACC
TTAAGCACTGTTTCGAGTCAGCGAAACAAGACTTAGCTCAAGGAAATGTGGTTGATGGTGAAGACTTTTTAAACGCCTTA
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J1MQ96

Antitoxin

Source ID Structure
AlphaFold DB A0A6B5NTK4

References