Detailed information of TA system TA163556

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tad-ata/COG5606-COG4679
• Location: 4701040..4701706
• Replicon: chromosome
• Accession: NZ_CP056466
• Organism: Citrobacter freundii strain RHBSTW-00486

Toxin (Protein)

• Gene name: tad
• Uniprot ID: -
• Locus tag: HV214_RS22815
• Protein ID: WP_046671413.1
• Coordinates: 4701347..4701706 (-)
• Length: 120 a.a.

Antitoxin (Protein)

• Gene name: ata
• Uniprot ID: A0A0D7LIR0
• Locus tag: HV214_RS22810
• Protein ID: WP_003844682.1
• Coordinates: 4701040..4701357 (-)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HV214_RS22780	4696714..4697538	+	825	WP_003031641.1	PTS system mannose/fructose/sorbose family transporter subunit IID	-
HV214_RS22785	4697606..4698829	+	1224	WP_003031639.1	L-sorbose 1-phosphate reductase	-
HV214_RS22790	4698831..4699643	+	813	WP_044702264.1	shikimate 5-dehydrogenase	-
HV214_RS22795	4699689..4700045	-	357	WP_046671411.1	hypothetical protein	-
HV214_RS22800	4700187..4700348	+	162	WP_003841416.1	phage protein	-
HV214_RS22805	4700752..4701030	+	279	WP_046671412.1	putative addiction module antidote protein	-
HV214_RS22810	4701040..4701357	-	318	WP_003844682.1	helix-turn-helix domain-containing protein	Antitoxin
HV214_RS22815	4701347..4701706	-	360	WP_046671413.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HV214_RS22820	4701920..4702609	+	690	WP_046671414.1	dipeptidase PepE	-
HV214_RS22825	4702675..4704306	-	1632	WP_003031618.1	Na/Pi cotransporter family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (14-106)

Antitoxin

• HigA2-like, helix-turn-helix domain (12-90)

Sequences

Toxin

Protein

• Length: 120 a.a.
• Molecular weight: 13451.52 Da
• Isoelectric Point: 10.2555

>T163556 WP_046671413.1 NZ_CP056466:c4701706-4701347 [Citrobacter freundii]
MTKPLYWVGQARKDLIAMPEHVRDTFGFALWLAQQGKQHSQTKPLKGFGGAGVLEVVEDYHGNAWRAVYTIQLKNAIYVL
HVFQKKSVSGKATPKPEIDLIYQRLKAAQRHAQESGYVT

Nucleotide

• Length: 360 bp

>T163556 NZ_CP072680:1424153-1424422 [Vibrio sp. SCSIO 43186]
ATGAGTAGTAGTCAACGTTTACTATCGTGGACTGATGACGCTTGGGATGACTACCTGTATTGGCAAACTCAAGACAAGAA
AACACTCAAGCGCATCAACAAACTCATCAATGATGTAAAGCGCTCCCCATTTGAGGGTATCGGTAAACCAGAGCCGTTAA
AAGAGAACTTATCTGGTTTTTGGTCTCGTCGTGTTGATGACACTAATAGGCTTGTTTACGCAGTCGATGATCAAGCGATA
ACGATAATCTCGTGTCGTTACCACTACTAA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11466.35 Da
• Isoelectric Point: 9.0005

>AT163556 WP_003844682.1 NZ_CP056466:c4701357-4701040 [Citrobacter freundii]
MSHDVETSSGNVYADLGAYDAEQMQLKAQLAMVIGEILQRKGLTQQQAAQLLGMTQPKLSNLLRGQFRGISEAKMLECLV
RLGRDVEIVVGKEGNMSGKLKVKFG

Nucleotide

• Length: 318 bp

>AT163556 NZ_CP072680:1423906-1424160 [Vibrio sp. SCSIO 43186]
ATGAGAATCGTATCTTTTACTGAAGCTAGAAATGGTCTTAAAGCTGTTTTGGACGGTGTAGTTAATGACGCTGATACAAC
AGTTATTACACGCCGTGATTCTGAAGATGCTGTGGTTATGTCTTTAGACTACTACAATAGCCTTATGGAGACCGTTCACT
TACTACGCTCTCCTCAAAACGCTGAACACCTAAACCGTTCGATAGCACAGTACCGCGCTGGTAAAACAACAACACGAGAG
TTAATTGATGAGTAG

Structures

Antitoxin

• AlphaFold DB: A0A0D7LIR0