163553

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yafW-ykfI/CbtA-CbeA
Location 3941185..3941864 Replicon chromosome
Accession NZ_CP056466
Organism Citrobacter freundii strain RHBSTW-00486

Toxin (Protein)


Gene name ykfI Uniprot ID A0A1B7JLK7
Locus tag HV214_RS19255 Protein ID WP_003031349.1
Coordinates 3941523..3941864 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name yafW Uniprot ID A0A1B7JLJ2
Locus tag HV214_RS19250 Protein ID WP_003031347.1
Coordinates 3941185..3941502 (+) Length 106 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HV214_RS19225 3937278..3939275 - 1998 WP_016245729.1 choline BCCT transporter BetT -
HV214_RS19230 3939779..3939970 + 192 Protein_3767 DUF905 family protein -
HV214_RS19235 3939984..3940445 + 462 WP_003031344.1 antirestriction protein -
HV214_RS19240 3940461..3940937 + 477 WP_003031345.1 RadC family protein -
HV214_RS19245 3940946..3941167 + 222 WP_003031346.1 DUF987 domain-containing protein -
HV214_RS19250 3941185..3941502 + 318 WP_003031347.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
HV214_RS19255 3941523..3941864 + 342 WP_003031349.1 type IV toxin-antitoxin system toxin YkfI Toxin
HV214_RS19265 3942494..3942958 - 465 WP_003843778.1 isoprenylcysteine carboxylmethyltransferase family protein -
HV214_RS19270 3943237..3944205 + 969 WP_000654812.1 IS5-like element IS903B family transposase -
HV214_RS19275 3944247..3944417 - 171 WP_181548249.1 hypothetical protein -
HV214_RS19280 3944407..3945423 + 1017 WP_001407551.1 IS5-like element IS5 family transposase -
HV214_RS19285 3945570..3946157 - 588 WP_003031365.1 fimbrillin MatB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 3899380..3947472 48092


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(5-104)


Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 12921.01 Da        Isoelectric Point: 9.6543

>T163553 WP_003031349.1 NZ_CP056466:3941523-3941864 [Citrobacter freundii]
MKTLPATTQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAVDILRARQATGLLRQSRNNVVR

Download         Length: 342 bp

>T163553 NZ_CP072680:c1404900-1404622 [Vibrio sp. SCSIO 43186]
ATGATTTTTTGGGAAGAAGAGTCTCTCAACGATCGTGAAAAGATCTTCGAATTTCTTTACGACTTCAACCCAGAGGCTGC
TGAAAAAACCGATGAAGTTATCGAAGCCAAAGTGGAGAACCTTCTAGAACAACCTCATATGGGAGTCCAACGTGACGGAA
TTAGAGGGCGATTGCTCATTATTCCTGAGATATCAATGATTGTTTCATACTGGATTGAAGGCGATATCATTCGCATTATG
CGTGTACTACATCAAAAACAAAAATTCCCTGCGAGCTAG

Antitoxin


Download         Length: 106 a.a.        Molecular weight: 12094.74 Da        Isoelectric Point: 7.0370

>AT163553 WP_003031347.1 NZ_CP056466:3941185-3941502 [Citrobacter freundii]
MSKITWGLQRDITPRLGARLVQEGNRLHYLADRASMTGKFSDIEYRKLDETFPHFIRQMESMLTTGELSTHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR

Download         Length: 318 bp

>AT163553 NZ_CP072680:c1405181-1404897 [Vibrio sp. SCSIO 43186]
ATGGACACCAGAATTCAGTTTCGTGTTGATGAAGAAACGAAGCGACTAGCTCAACAAATGGCAGAAAGCCAAGGCAGAAC
ACTAAGCGACGCTTGTCGTGAACTGACTGAACAACTAGCTGACCAACAGAGAAAGACTTTATCTCACGATGCATGGCTAA
CTGAACAAGTTAACTTGGCTTTTGAGAAGTTTGACTTAGGAAAAGCCAACTTTGTTGATCACGAATCAGCTAAATCACGT
ATGGCTGAGCGCAAAGCAAAAATCCGTAACCGAGGCAAAGTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1B7JLK7

Antitoxin

Source ID Structure
AlphaFold DB A0A1B7JLJ2

References